Real-world EGFR testing practices for non-small-cell lung cancer by thoracic pathology laboratories across Europe.

Autor:	Hofman P; Laboratory of Clinical and Experimental Pathology, FHU OncoAge, Biobank Côte d'Azur BB-0033-00025, Louis Pasteur Hospital, IRCAN, Université Côte d'Azur, Nice, France. Electronic address: hofman.p@chu-nice.fr., Calabrese F; Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, Padova, Italy., Kern I; Department of Pathology, University Clinic Golnik, Golnik, Slovenia., Adam J; Department of Pathology, Groupe Hospitalier Paris Saint-Joseph, Paris, France., Alarcão A; IAP-PM, Institute of Anatomical and Molecular Pathology, Faculty of Medicine, University of Coimbra, 3004-504 Coimbra, Portugal., Alborelli I; Department of Pathology, Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel, Basel, Switzerland., Anton NT; Department of Genetics, University Hospital Bichat-Claude Bernard, Paris University, Paris, France., Arndt A; Institute of Pathology and Molecular Pathology, Bundeswehrkrankenhaus Ulm, Oberer Eselsberg 40, 89081 Ulm, Germany., Avdalyan A; Multidisciplinary Clinical Center 'Kommunarka' of the Moscow Health Department, Moscow, Russia., Barberis M; Oncogenomics Unit, European Institute of Oncology (IEO), Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy., Bégueret H; Department of Pathology, University Hospital of Bordeaux, Hôpital Haut-Lévêque, Pessac, France., Bisig B; Institute of Pathology, Department of Laboratory Medicine and Pathology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland., Blons H; Pharmacogenomics and Molecular Oncology Unit, Biochemistry Department, Assistance Publique-Hopitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France., Boström P; Department of Pathology, Turku University Hospital, Turku, Finland., Brcic L; Diagnostic and Research Institute of Pathology, Medical University of Graz, Graz, Austria., Bubanovic G; Laboratory for Molecular Pathology, Department of Pathology, University of Zagreb School of Medicine and University Hospital Centre Zagreb, Zagreb, Croatia., Buisson A; Department of Biopathology, Centre Léon Bérard, Lyon, France., Caliò A; Department of Diagnostics and Public Health, Section of Pathology, University and Hospital Trust of Verona, Verona, Italy., Cannone M; Inter-Hospital Pathology Division, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), MultiMedica, Milan, Italy., Carvalho L; IAP-PM, Institute of Anatomical and Molecular Pathology, Faculty of Medicine, University of Coimbra, 3004-504 Coimbra, Portugal., Caumont C; Department of Tumor Biology, University Hospital of Bordeaux, Hospital Haut-Lévêque, Pessac, France., Cayre A; Department of Biopathology, Jean Perrin Centre, Clermont-Ferrand, France., Chalabreysse L; Department of Pathology, Groupement Hospitalier Est, Bron, France., Chenard MP; Department of Pathology, University Hospital of Strasbourg, 67098 Strasbourg, France., Conde E; Department of Pathology, 12 de Octubre University Hospital, Universidad Complutense de Madrid, Research Institute 12 de Octubre University Hospital (i+12), CIBERONC, Madrid, Spain., Copin MC; Department of Pathology, Université d'Angers, Centre Hospitalier Universitaire d'Angers, Angers, France., Côté JF; Department of Pathology, Institut Mutualiste Montsouris, Paris, France., D'Haene N; Department of Pathology, Erasme Hospital, HUB ULB, Brussels, Belgium., Dai HY; Department of Pathology, St. Olav's Hospital, Trondheim University Hospital, Trondheim, Norway., de Leval L; Institute of Pathology, Department of Laboratory Medicine and Pathology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland., Delongova P; Institute of Molecular and Clinical Pathology and Medical Genetics, Faculty of Medicine, University Hospital Ostrava, Ostrava, Czech Republic., Denčić-Fekete M; Institute for Pathology Medical Faculty, Belgrade, Serbia., Fabre A; Department of Histopathology, St. Vincent's University Hospital, University College Dublin School of Medicine, Dublin, Ireland., Ferenc F; Department of Pathology, University of Oradea, Oradea, Romania., Forest F; Department of Pathology, University Hospital of Saint-Etienne, Saint-Etienne, France., de Fraipont F; Medical Unit of Molecular Genetic (Hereditary Diseases and Oncology), Grenoble University Hospital, Grenoble, France., Garcia-Martos M; Department of Pathology, Gregorio Marañón General University Hospital, Madrid, Spain., Gauchotte G; Department of Biopathology, CHRU-ICL, CHRU Nancy, Vandoeuvre-lès-Nancy, France., Geraghty R; Department of Histopathology, St. Vincent's University Hospital, University College Dublin School of Medicine, Dublin, Ireland., Guerin E; Department of Molecular Cancer Genetics, Laboratory of Biochemistry and Molecular Biology, University Hospital of Strasbourg, Strasbourg, France., Guerrero D; Biomedical Research Centre, Navarra Health Service, Pamplona, Navarra, Spain., Hernandez S; Department of Pathology, 12 de Octubre University Hospital, Universidad Complutense de Madrid, Research Institute 12 de Octubre University Hospital (i+12), CIBERONC, Madrid, Spain., Hurník P; Institute of Molecular and Clinical Pathology and Medical Genetics, Faculty of Medicine, University Hospital Ostrava, Ostrava, Czech Republic., Jean-Jacques B; Department of Pathology, CHU de Caen Côte de Nacre, Caen, France., Kashofer K; Diagnostic and Research Institute of Pathology, Medical University of Graz, Graz, Austria., Kazdal D; Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany; Translational Lung Research Center Heidelberg (TLRC-H), member of the German Center for Lung Research (DZL), Heidelberg, Germany., Lantuejoul S; Department of Biopathology, Centre Leon Berard Unicancer and Pathology Research Platform, Cancer Research Center of Lyon (CRCL), Lyon, France., Leonce C; Department of Pathology, Groupement Hospitalier Est, Bron, France., Lupo A; Department of Pathology, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Université de Paris, Paris, France., Malapelle U; Department of Public Health, University of Naples Federico II, Naples, Italy., Matej R; Department of Pathology and Molecular Medicine, Thomayer University Hospital, Prague, Czech Republic., Merlin JL; Department of Biopathology, Institut de Cancérologie de Lorraine, University of Lorraine, Vandoeuvre-Les-Nancy, France., Mertz KD; Institute of Pathology, Cantonal Hospital Baselland, Liestal, Switzerland., Morel A; Department of Innate Immunity and Immunotherapy, Institut de Cancérologie de l'Ouest - Centre Paul Papin, Angers, France., Mutka A; HUSLAB, Department of Pathology, Helsinki University Hospital, Helsinki, Finland., Normanno N; Cell Biology and Biotherapy Unit, INT-Fondazione Pascale, Via M. Semmola, Naples, Italy., Ovidiu P; Department of Pathology, University of Oradea, Oradea, Romania., Panizo A; Department of Pathology, Complejo Hospitalario de Navarra, Pamplona, Navarra, Spain., Papotti MG; Division of Pathology, University Hospital Città Della Salute, Turin, Italy., Parobkova E; Department of Pathology and Molecular Medicine, Thomayer University Hospital, Prague, Czech Republic., Pasello G; Division of Medical Oncology 2, Veneto Institute of Oncology IOV-IRCCS, Padova, Italy., Pauwels P; Department of Pathology, University Hospital Antwerp and University of Antwerp, Antwerp, Belgium., Pelosi G; Department of Oncology and Hemato-Oncology, University of Milan, Milan, Italy., Penault-Llorca F; Department of Pathology, Clermont Auvergne University, 'Molecular Imaging and Theranostic Strategies', Center Jean Perrin, Montalembert, Clermont-Ferrand, France., Picot T; Department of Pathology, University Hospital of Saint-Etienne, Saint-Etienne, France., Piton N; Department of Pathology, Rouen University Hospital, France and Normandie University, UNIROUEN, Inserm U1245, Rouen, France., Pittaro A; Division of Pathology, University Hospital Città Della Salute, Turin, Italy., Planchard G; Department of Pathology, CHU de Caen Côte de Nacre, Caen, France., Poté N; Department of Pathology, Hospital Bichat Bichat, Assistance Publique Hôpitaux de Paris; Université Paris Cité, Paris, France., Radonic T; Department of Pathology, Amsterdam University Medical Center, VUMC, University of Amsterdam, Amsterdam, Netherlands., Rapa I; Pathology Unit, San Luigi Hospital, Orbassano Turin, Italy., Rappa A; Oncogenomics Unit, European Institute of Oncology (IEO), Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy., Roma C; Cell Biology and Biotherapy Unit, INT-Fondazione Pascale, Via M. Semmola, Naples, Italy., Rot M; Department of Pathology, University Clinic Golnik, Golnik, Slovenia., Sabourin JC; Department of Pathology, Rouen University Hospital, France and Normandie University, UNIROUEN, Inserm U1245, Rouen, France., Salmon I; Department of Pathology, Erasme Hospital, HUB ULB, Brussels, Belgium; CurePath, Jumet, Belgium., Prince SS; Department of Pathology, Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel, Basel, Switzerland., Scarpa A; Department of Diagnostics and Public Health, Section of Pathology, University and Hospital Trust of Verona, Verona, Italy., Schuuring E; Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, Netherlands., Serre I; Department of Pathology, Gui de Chauliac Hospital, Montpellier University Medical Center, University of Montpellier, 80 Avenue Augustin Fliche, Montpellier, France., Siozopoulou V; Department of Pathology, University Hospital Antwerp and University of Antwerp, Antwerp, Belgium., Sizaret D; Department of Pathology, CHRU Tours - Hôpital Trousseau, Chambray-lès-Tours, France., Smojver-Ježek S; Division for Pulmonary Cytology, Department of Pathology and Cytology, University Hospital Centre Zagreb, School of Medicine, University of Zagreb, Zagreb, Croatia., Solassol J; Solid Tumour Laboratory, Pathology and Oncobiology Department, CHU Montpellier, University of Montpellier, Montpellier, France., Steinestel K; Institute of Pathology and Molecular Pathology, Bundeswehrkrankenhaus Ulm, Oberer Eselsberg 40, 89081 Ulm, Germany., Stojšić J; Department of Thoracic Pathology, Section of Pathology, University Clinical Centre of Serbia, Belgrade, Serbia., Syrykh C; Department of Pathology, IUC-T-Oncopole, Toulouse, France., Timofeev S; Multidisciplinary Clinical Center 'Kommunarka' of the Moscow Health Department, Moscow, Russia., Troncone G; Department of Pathology, University of Oradea, Oradea, Romania., Uguen A; Department of Pathological Anatomy and Cytology, CHRU de Brest, Brest, France; LBAI, UMR1227, INSERM, University of Brest, CHU de Brest, Brest, France., Valmary-Degano S; Department of Pathology, Institute for Advanced Biosciences, CHU Grenoble Alpes, Université Grenoble Alpes, Grenoble, France., Vigier A; Department of Pathology, IUC-T-Oncopole, Toulouse, France., Volante M; Department of Oncology, University of Turin, San Luigi Hospital, Orbassano, Turin, Italy., Wahl SGF; Department of Pathology, St. Olav's Hospital, Trondheim University Hospital, Trondheim, Norway., Stenzinger A; Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany; Translational Lung Research Center Heidelberg (TLRC-H), member of the German Center for Lung Research (DZL), Heidelberg, Germany., Ilié M; Laboratory of Clinical and Experimental Pathology, FHU OncoAge, Biobank Côte d'Azur BB-0033-00025, Louis Pasteur Hospital, IRCAN, Université Côte d'Azur, Nice, France.
Jazyk:	angličtina
Zdroj:	ESMO open [ESMO Open] 2023 Oct; Vol. 8 (5), pp. 101628. Date of Electronic Publication: 2023 Sep 14.
DOI:	10.1016/j.esmoop.2023.101628
Abstrakt:	Background: Testing for epidermal growth factor receptor (EGFR) mutations is an essential recommendation in guidelines for metastatic non-squamous non-small-cell lung cancer, and is considered mandatory in European countries. However, in practice, challenges are often faced when carrying out routine biomarker testing, including access to testing, inadequate tissue samples and long turnaround times (TATs). Materials and Methods: To evaluate the real-world EGFR testing practices of European pathology laboratories, an online survey was set up and validated by the Pulmonary Pathology Working Group of the European Society of Pathology and distributed to 64 expert testing laboratories. The retrospective survey focussed on laboratory organisation and daily EGFR testing practice of pathologists and molecular biologists between 2018 and 2021. Results: TATs varied greatly both between and within countries. These discrepancies may be partly due to reflex testing practices, as 20.8% of laboratories carried out EGFR testing only at the request of the clinician. Many laboratories across Europe still favour single-test sequencing as a primary method of EGFR mutation identification; 32.7% indicated that they only used targeted techniques and 45.1% used single-gene testing followed by next-generation sequencing (NGS), depending on the case. Reported testing rates were consistent over time with no significant decrease in the number of EGFR tests carried out in 2020, despite the increased pressure faced by testing facilities during the COVID-19 pandemic. ISO 15189 accreditation was reported by 42.0% of molecular biology laboratories for single-test sequencing, and by 42.3% for NGS. 92.5% of laboratories indicated they regularly participate in an external quality assessment scheme. Conclusions: These results highlight the strong heterogeneity of EGFR testing that still occurs within thoracic pathology and molecular biology laboratories across Europe. Even among expert testing facilities there is variability in testing capabilities, TAT, reflex testing practice and laboratory accreditation, stressing the need to harmonise reimbursement technologies and decision-making algorithms in Europe. (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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