A novel pectin methylesterase inhibitor, PMEI3, in common bean suggests a key role of pectin methylesterification in Pseudomonas resistance.

Autor:	De la Rubia AG; Área de Fisiología Vegetal, Dpto Ingenieria y Ciencias Agrarias, Universidad de León, León, E-24071, Spain.; Department of Biology, ETH Zurich, 8092 Zurich, Switzerland., Largo-Gosens A; Área de Fisiología Vegetal, Dpto Ingenieria y Ciencias Agrarias, Universidad de León, León, E-24071, Spain.; Centro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370146, Chile., Yusta R; Centro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370146, Chile.; ANID - Millennium Science Initiative Program - Millennium Institute Center for Genome Regulation (CRG), 7800003, Santiago, Chile., Sepúlveda-Orellana P; Centro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370146, Chile., Riveros A; Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, 8331150, Chile.; ANID - Millennium Science Initiative Program - Millennium Nucleus for the Development of Super Adaptable Plants (MN-SAP), Santiago, Chile., Centeno ML; Área de Fisiología Vegetal, Dpto Ingenieria y Ciencias Agrarias, Universidad de León, León, E-24071, Spain., Sanhueza D; Centro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370146, Chile., Meneses C; ANID - Millennium Science Initiative Program - Millennium Institute Center for Genome Regulation (CRG), 7800003, Santiago, Chile.; Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, 8331150, Chile.; ANID - Millennium Science Initiative Program - Millennium Nucleus for the Development of Super Adaptable Plants (MN-SAP), Santiago, Chile.; Departamento de Fruticultura y Enología, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, 7820436, Chile., Saez-Aguayo S; Centro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8370146, Chile.; ANID - Millennium Science Initiative Program - Millennium Nucleus for the Development of Super Adaptable Plants (MN-SAP), Santiago, Chile.; Chilean fruits cell wall Components as Biotechnological resources (CHICOBIO), Proyecto Anillo ACT210025, Santiago, Chile., García-Angulo P; Área de Fisiología Vegetal, Dpto Ingenieria y Ciencias Agrarias, Universidad de León, León, E-24071, Spain.
Jazyk:	angličtina
Zdroj:	Journal of experimental botany [J Exp Bot] 2024 Jan 01; Vol. 75 (1), pp. 364-390.
DOI:	10.1093/jxb/erad362
Abstrakt:	The mechanisms underlying susceptibility to and defense against Pseudomonas syringae (Pph) of the common bean (Phaseolus vulgaris) have not yet been clarified. To investigate these, 15-day-old plants of the variety Riñón were infected with Pph and the transcriptomic changes at 2 h and 9 h post-infection were analysed. RNA-seq analysis showed an up-regulation of genes involved in defense/signaling at 2 h, most of them being down-regulated at 9 h, suggesting that Pph inhibits the transcriptomic reprogramming of the plant. This trend was also observed in the modulation of 101 cell wall-related genes. Cell wall composition changes at early stages of Pph infection were associated with homogalacturonan methylation and the formation of egg boxes. Among the cell wall genes modulated, a pectin methylesterase inhibitor 3 (PvPMEI3) gene, closely related to AtPMEI3, was detected. PvPMEI3 protein was located in the apoplast and its pectin methylesterase inhibitory activity was demonstrated. PvPMEI3 seems to be a good candidate to play a key role in Pph infection, which was supported by analysis of an Arabidopsis pmei3 mutant, which showed susceptibility to Pph, in contrast to resistant Arabidopsis Col-0 plants. These results indicate a key role of the degree of pectin methylesterification in host resistance to Pph during the first steps of the attack. (© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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