Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analyses.

Autor:	Gstöttenmayer F; Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria., Moyaba P; Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria - Epidemiology, Parasites and Vectors, Agricultural Research Council-Onderstepoort Veterinary Research, 100 Soutpan Road, Private Bag X05, Onderstepoort, 0110 South Africa., Rodriguez M; Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria., Mulandane FC; University Eduardo Mondlane, Centro de Biotecnologia, Av. de Moçambique Km 1.5, Maputo, Mozambique., Mucache HN; University Eduardo Mondlane, Centro de Biotecnologia, Av. de Moçambique Km 1.5, Maputo, Mozambique., Neves L; University Eduardo Mondlane, Centro de Biotecnologia, Av. de Moçambique Km 1.5, Maputo, Mozambique - University of Pretoria, Department of Veterinary Tropical Diseases, Private Bag X04, Onderstepoort 0110, South Africa., De Beer C; Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria., Ravel S; University of Montpellier, Cirad, IRD, Intertryp, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France., De Meeûs T; University of Montpellier, Cirad, IRD, Intertryp, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France., Mach RL; Institute of Chemical, Environmental, and Bioscience Engineering, Vienna University of Technology, Gumpendorfer Straße 1a, 1060 Vienna, Austria., Vreysen MJB; Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria., Abd-Alla AMM; Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria.
Jazyk:	angličtina
Zdroj:	Parasite (Paris, France) [Parasite] 2023; Vol. 30, pp. 34. Date of Electronic Publication: 2023 Sep 15.
DOI:	10.1051/parasite/2023038
Abstrakt:	Tsetse flies, the vectors of African trypanosomes are of key medical and economic importance and one of the constraints for the development of Africa. Tsetse fly control is one of the most effective and sustainable strategies used for controlling the disease. Knowledge about population structure and level of gene flow between neighbouring populations of the target vector is of high importance to develop appropriate strategies for implementing effective management programmes. Microsatellites are commonly used to identify population structure and assess dispersal of the target populations and have been developed for several tsetse species but were lacking for Glossina brevipalpis. In this study, we screened the genome of G. brevipalpis to search for suitable microsatellite markers and nine were found to be efficient enough to distinguish between different tsetse populations. The availability of these novel microsatellite loci will help to better understand the population biology of G. brevipalpis and to assess the level of gene flow between different populations. Such information will help with the development of appropriate strategies to implement the sterile insect technique (SIT) in the framework of an area-wide integrated pest management (AW-IPM) approach to manage tsetse populations and ultimately address the trypanosomoses problem in these targeted areas. (© F. Gstöottenmayer et al., published by EDP Sciences, 2023.)
Databáze:	MEDLINE
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