| Autor: |
Daphnis T; Institute of Condensed Matter and Nanoscience, Université catholique de Louvain, 1 Place Louis Pasteur, 1348 Louvain-la-Neuve, Belgium., Tomasetti B; Institute of Condensed Matter and Nanoscience, Université catholique de Louvain, 1 Place Louis Pasteur, 1348 Louvain-la-Neuve, Belgium., Delmez V; Institute of Condensed Matter and Nanoscience, Université catholique de Louvain, 1 Place Louis Pasteur, 1348 Louvain-la-Neuve, Belgium., Vanvarenberg K; Louvain Drug Research Institute, Université catholique de Louvain, Avenue Mounier 73, 1200 Brussels, Belgium., Préat V; Louvain Drug Research Institute, Université catholique de Louvain, Avenue Mounier 73, 1200 Brussels, Belgium., Thieffry C; Institut De Duve, Université catholique de Louvain, Avenue Hippocrate 75, 1200 Brussels, Belgium., Henriet P; Institut De Duve, Université catholique de Louvain, Avenue Hippocrate 75, 1200 Brussels, Belgium., Dupont-Gillain C; Institute of Condensed Matter and Nanoscience, Université catholique de Louvain, 1 Place Louis Pasteur, 1348 Louvain-la-Neuve, Belgium., Delcorte A; Institute of Condensed Matter and Nanoscience, Université catholique de Louvain, 1 Place Louis Pasteur, 1348 Louvain-la-Neuve, Belgium. |
| Abstrakt: |
The potential of mass spectrometry imaging, and especially ToF-SIMS 2D and 3D imaging, for submicrometer-scale, label-free molecular localization in biological tissues is undisputable. Nevertheless, sensitivity issues remain, especially when one wants to achieve the best lateral and vertical (nanometer-scale) resolution. In this study, the interest of in situ matrix transfer for tissue analysis with cluster ion beams (Bi n + , Ar n + ) is explored in detail, using a series of six low molecular weight acidic (MALDI) matrices. After estimating the sensitivity enhancements for phosphatidylcholine (PC), an abundant lipid type present in almost any kind of cell membrane, the most promising matrices were softly transferred in situ on mouse brain and human uterine tissue samples using a 10 keV Ar 3000 + cluster beam. Signal enhancements up to 1 order of magnitude for intact lipid signals were observed in both tissues under Bi 5 + and Ar 3000 + bombardment. The main findings of this study lie in the in-depth characterization of uterine tissue samples, the demonstration that the transferred matrices also improve signal efficiency in the negative ion polarity and that they perform as well when using Bi n + and Ar n + primary ions for analysis and imaging. |
