Analysis of the Immunostimulatory Effects of Cytokine-Expressing Internal Ribosome Entry Site-Based RNA Adjuvants and Their Applications.

Autor:	Lee YS; Department of Biotechnology.; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon., Bang YJ; Department of Biotechnology.; Central Research Institute, SML Biopharm, Gwangmyeong., Yoo S; Center for Brain Technology, Brain Science Institute, Korea Institute of Science and Technology, Seoul., Park SI; Central Research Institute, SML Biopharm, Gwangmyeong., Park HJ; Department of Biotechnology.; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon., Kwak HW; Central Research Institute, SML Biopharm, Gwangmyeong., Bae SH; Department of Biotechnology.; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon., Park HJ; Central Research Institute, SML Biopharm, Gwangmyeong., Kim JY; Department of Biotechnology.; Central Research Institute, SML Biopharm, Gwangmyeong., Youn SB; Department of Biotechnology.; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon., Roh G; Department of Biotechnology.; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon., Lee S; Department of Biotechnology.; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon., Kwon SP; Center for Brain Technology, Brain Science Institute, Korea Institute of Science and Technology, Seoul., Bang EK; Center for Brain Technology, Brain Science Institute, Korea Institute of Science and Technology, Seoul., Keum G; Center for Brain Technology, Brain Science Institute, Korea Institute of Science and Technology, Seoul., Nam JH; BK21 FOUR Department of Biotechnology, The Catholic University of Korea, Bucheon.; Department of Medical and Biological Sciences, The Catholic University of Korea, Bucheon., Hong SH; Department of Microbiology, College of Medicine, Ewha Womans University, Seoul, Republic of Korea.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2024 May 15; Vol. 229 (5), pp. 1408-1418.
DOI:	10.1093/infdis/jiad392
Abstrakt:	Developing new adjuvants that can effectively induce humoral and cellular immune responses while broadening the immune response is of great value. In this study, we aimed to develop single-stranded RNA adjuvants expressing (1) granulocyte monocyte colony-stimulating factor or (2) interleukin 18 based on the encephalomyocarditis virus internal ribosome entry site; we also tested their efficacy in combination with ovalbumin or inactivated influenza vaccines. Notably, cytokine-expressing RNA adjuvants increased the expression of antigen-presenting cell activation markers in mice. Specifically, when combined with ovalbumin, RNA adjuvants expressing granulocyte monocyte colony-stimulating factor increased CD4+ T-cell responses, while those expressing interleukin 18 increased CD8+ T-cell responses. Cytokine-expressing RNA adjuvants further increased the frequency of polyclonal T cells with the influenza vaccine and reduced the clinical illness scores and weight loss of mice after viral challenge. Collectively, our results suggest that cytokine-expressing RNA adjuvants can be applied to protein-based or inactivated vaccines to increase their efficacy. Competing Interests: Potential conflicts of interest. All authors: No reported conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. (© The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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