One-step selective layer assemble: A versatile approach for the development of a SARS-CoV-2 electrochemical immunosensor.

Autor: Martins G; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CEP 81531-980, Curitiba, PR, Brazil., Galeski HR; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CEP 81531-980, Curitiba, PR, Brazil., Andrade GA; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CEP 81531-980, Curitiba, PR, Brazil., Valenga MGP; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CEP 81531-980, Curitiba, PR, Brazil., Ramos MK; Grupo de Química de Materiais (GQM), Departamento de Química, Universidade Federal do Paraná (UFPR), CEP 81531-980, Curitiba, PR, Brazil., Zarbin AJG; Grupo de Química de Materiais (GQM), Departamento de Química, Universidade Federal do Paraná (UFPR), CEP 81531-980, Curitiba, PR, Brazil., Janegitz BC; Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, 13600-970, Araras, São Paulo, Brazil., Müller-Santos M; Núcleo de Fixação de Nitrogênio (NFIX), Departamento de Bioquímica e Biologia Molecular, Universidade Federal Do Paraná (UFPR), CP: 19046, CEP: 81531-980, Curitiba, PR, Brazil., de Souza EM; Núcleo de Fixação de Nitrogênio (NFIX), Departamento de Bioquímica e Biologia Molecular, Universidade Federal Do Paraná (UFPR), CP: 19046, CEP: 81531-980, Curitiba, PR, Brazil., Marcolino-Junior LH; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CEP 81531-980, Curitiba, PR, Brazil. Electronic address: luiz1berto@ufpr.br., Bergamini MF; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal do Paraná (UFPR), CEP 81531-980, Curitiba, PR, Brazil. Electronic address: bergamini@ufpr.br.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2023 Oct 16; Vol. 1278, pp. 341726. Date of Electronic Publication: 2023 Aug 16.
DOI: 10.1016/j.aca.2023.341726
Abstrakt: The appearance of new viruses and diseases has made the development of rapid and reliable diagnostic tests crucial. In light of it, we proposed a new method for assembling an electrochemical immunosensor, based on a one-step approach for selective layer formation. For this purpose, a mixture containing the immobilizing agent (polyxydroxybutyrate, PHB) and the recognition element (antibodies against SARS-CoV-2 nucleocapsid protein) was prepared and used to modify a screen-printed carbon electrode with electrodeposited graphene oxide, for the detection of SARS-CoV-2 nucleocapsid protein (N-protein). Under optimum conditions, N-protein was successfully detected in three different matrixes - saliva, serum, and nasal swab, with the lowest detectable values of 50 pg mL -1 , 1.0 ng mL -1, and 50 pg mL -1 , respectively. Selectivity was assessed against SARS-CoV-2 receptor-binding domain protein (RBD) and antibodies against yellow fever (YF), and no significant response was observed in presence of interferents, reinforcing the suitability of the proposed one-step approach for selective layer formation. The proposed biosensor was stable for up to 14 days, and the mixture was suitable for immunosensor preparation even after 60 days of preparation. The proposed assembly strategy reduces the cost, analysis time, and waste generation. This reduction is achieved through miniaturization, which results in the decreased use of reagents and sample volumes. Additionally, this approach enables healthcare diagnostics to be conducted in developing regions with limited resources. Therefore, the proposed one-step approach for selective layer formation is a suitable, simpler, and a reliable alternative for electrochemical immunosensing.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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