Kidney morphology and renal expression of aquaporins 2, 3 and 4 during cerulein - Induced chronic pancreatitis in pigs.

Autor: Michałek K; Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology in Szczecin, Poland. Electronic address: kmichalek@zut.edu.pl., Oberska P; Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology in Szczecin, Poland., Murawski M; Department of Animal Nutrition, Biotechnology and Fisheries, University of Agriculture in Kraków, Poland., Schwarz T; Department of Animal Genetics, Breeding and Ethology, University of Agriculture in Kraków, Poland., Tomaszewska E; Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Poland., Muszyński S; Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, Poland., Świątkiewicz M; National Research Institute of Animal Production, Department of Animal Nutrition and Feed Science Balice, Poland., Korytkowski Ł; National Research Institute of Animal Production, Department of Reproductive Biotechnology and Cryoconservation, Balice, Poland., Bonior J; Department of Medical Physiology, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, Cracow, Poland., Zelent M; Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology in Szczecin, Poland., Ayomide DS; Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology in Szczecin, Poland., Grabowska M; Department of Histology and Developmental Biology, Faculty of Health Sciences, Pomeranian Medical University, Szczecin, Poland.
Jazyk: angličtina
Zdroj: Advances in medical sciences [Adv Med Sci] 2023 Sep; Vol. 68 (2), pp. 306-313. Date of Electronic Publication: 2023 Sep 13.
DOI: 10.1016/j.advms.2023.09.002
Abstrakt: Purpose: Chronic pancreatitis (CP) is associated with serious complications and reduced quality of life. Kidney failure is a frequent complication of acute pancreatitis (AP), however limited information is available regarding the impact of CP on this condition. In the kidney, 9 aquaporins (AQPs) are expressed to maintain body water homeostasis and concentrate urine. The purpose of this study was to morphologically assess and analyze the location and expression of AQP2, AQP3 and AQP4 and determine whether CP affects renal structure and expression of AQPs in collecting duct (CD) principal cells.
Materials/methods: CP was induced in domestic pigs through intramuscular injections of cerulein (1 ​μg/kg ​bw/day for 6 days; n ​= ​5); pigs without CP (n ​= ​5) were used as a control group. Kidney samples were collected 6 weeks after the last injection and subjected to histological examination. Expression of AQPs was determined by immunohistochemistry and Western blot.
Results: The kidneys of animals with CP exhibited moderate changes, including glomerular enlargement, increased collagen percentage, numerous stromal erythrorrhages and inflammatory infiltrations compared to control group. Although the total abundance of AQP2 in the CD decreased in pigs after cerulein administration, the difference was not statistically significant. Expression of AQP3 and AQP4 was limited to the basolateral membrane of the CD cells. AQP4 abundance remained relatively stable in both groups, while AQP3 expression increased nearly three-fold in pigs with CP.
Conclusion: This study identified morphological alterations and a statistically significant increase in the expression of renal AQP3 when pigs developed CP.
Competing Interests: Declaration of competing interest The authors declare no conflict of interests.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: