δ-Catenin controls astrocyte morphogenesis via layer-specific astrocyte-neuron cadherin interactions.

Autor:	Tan CX; Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA., Bindu DS; Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA., Hardin EJ; Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA., Sakers K; Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA., Baumert R; Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA., Ramirez JJ; Department of Neurobiology, Duke University School of Medicine, Durham, NC, USA., Savage JT; Department of Neurobiology, Duke University School of Medicine, Durham, NC, USA., Eroglu C; Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA.; Department of Neurobiology, Duke University School of Medicine, Durham, NC, USA.; Howard Hughes Medical Institute, Duke University School of Medicine , Durham, NC, USA.
Jazyk:	angličtina
Zdroj:	The Journal of cell biology [J Cell Biol] 2023 Nov 06; Vol. 222 (11). Date of Electronic Publication: 2023 Sep 14.
DOI:	10.1083/jcb.202303138
Abstrakt:	Astrocytes control the formation of specific synaptic circuits via cell adhesion and secreted molecules. Astrocyte synaptogenic functions are dependent on the establishment of their complex morphology. However, it is unknown if distinct neuronal cues differentially regulate astrocyte morphogenesis. δ-Catenin was previously thought to be a neuron-specific protein that regulates dendrite morphology. We found δ-catenin is also highly expressed by astrocytes and required both in astrocytes and neurons for astrocyte morphogenesis. δ-Catenin is hypothesized to mediate transcellular interactions through the cadherin family of cell adhesion proteins. We used structural modeling and biochemical analyses to reveal that δ-catenin interacts with the N-cadherin juxtamembrane domain to promote N-cadherin surface expression. An autism-linked δ-catenin point mutation impaired N-cadherin cell surface expression and reduced astrocyte complexity. In the developing mouse cortex, only lower-layer cortical neurons express N-cadherin. Remarkably, when we silenced astrocytic N-cadherin throughout the cortex, only lower-layer astrocyte morphology was disrupted. These findings show that δ-catenin controls astrocyte-neuron cadherin interactions that regulate layer-specific astrocyte morphogenesis. (© 2023 Tan et al.)
Databáze:	MEDLINE
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