Blue-green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers.

Autor: Kanawati B; Analytical BioGeoChemistry Helmholtz Zentrum München Neuherberg Germany., Bertic M; Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology Helmholtz Zentrum München Neuherberg Germany., Moritz F; Analytical BioGeoChemistry Helmholtz Zentrum München Neuherberg Germany., Habermann F; Institute of Anatomy, Histology and Embryology, Department of Veterinary Sciences Ludwig-Maximilians-University Munich Munich Germany., Zimmer I; Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology Helmholtz Zentrum München Neuherberg Germany., Mackey D; Department of Horticulture and Crop Science and Department of Molecular Genetics Ohio State University Columbus Ohio USA., Schmitt-Kopplin P; Analytical BioGeoChemistry Helmholtz Zentrum München Neuherberg Germany., Schnitzler JP; Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology Helmholtz Zentrum München Neuherberg Germany., Durner J; Institute of Biochemical Plant Pathology Helmholtz Zentrum München Neuherberg Germany., Gaupels F; Institute of Biochemical Plant Pathology Helmholtz Zentrum München Neuherberg Germany.
Jazyk: angličtina
Zdroj: Plant direct [Plant Direct] 2023 Sep 12; Vol. 7 (9), pp. e531. Date of Electronic Publication: 2023 Sep 12 (Print Publication: 2023).
DOI: 10.1002/pld3.531
Abstrakt: Infection of Arabidopsis with avirulent Pseudomonas syringae and exposure to nitrogen dioxide (NO 2 ) both trigger hypersensitive cell death (HCD) that is characterized by the emission of bright blue-green (BG) autofluorescence under UV illumination. The aim of our current work was to identify the BG fluorescent molecules and scrutinize their biosynthesis, localization, and functions during the HCD. Compared with wild-type (WT) plants, the phenylpropanoid-deficient mutant fah1 developed normal HCD except for the absence of BG fluorescence. Ultrahigh resolution metabolomics combined with mass difference network analysis revealed that WT but not fah1 plants rapidly accumulate dehydrodimers of sinapic acid, sinapoylmalate, 5-hydroxyferulic acid, and 5-hydroxyferuloylmalate during the HCD. FAH1-dependent BG fluorescence appeared exclusively within dying cells of the upper epidermis as detected by microscopy. Saponification released dehydrodimers from cell wall polymers of WT but not fah1 plants. Collectively, our data suggest that HCD induction leads to the formation of free BG fluorescent dehydrodimers from monomeric sinapates and 5-hydroxyferulates. The formed dehydrodimers move from upper epidermis cells into the apoplast where they esterify cell wall polymers. Possible functions of phenylpropanoid dehydrodimers are discussed.
Competing Interests: No conflict of interest declared.
(© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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