Sox11 is enriched in myogenic progenitors but dispensable for development and regeneration of the skeletal muscle.

Autor: Oprescu SN; Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA., Baumann N; Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA., Chen X; Department of Animal Sciences, Purdue University, West Lafayette, IN, 47907, USA., Sun Q; Department of Orthopedics and Traumatology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Hong Kong, China., Zhao Y; Department of Orthopedics and Traumatology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Hong Kong, China., Yue F; Department of Animal Sciences, Purdue University, West Lafayette, IN, 47907, USA., Wang H; Department of Orthopedics and Traumatology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Hong Kong, China., Kuang S; Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA. skuang@purdue.edu.; Department of Animal Sciences, Purdue University, West Lafayette, IN, 47907, USA. skuang@purdue.edu.; Center for Cancer Research, Purdue University, West Lafayette, IN, 47907, USA. skuang@purdue.edu.
Jazyk: angličtina
Zdroj: Skeletal muscle [Skelet Muscle] 2023 Sep 13; Vol. 13 (1), pp. 15. Date of Electronic Publication: 2023 Sep 13.
DOI: 10.1186/s13395-023-00324-0
Abstrakt: Transcription factors (TFs) play key roles in regulating differentiation and function of stem cells, including muscle satellite cells (MuSCs), a resident stem cell population responsible for postnatal regeneration of the skeletal muscle. Sox11 belongs to the Sry-related HMG-box (SOX) family of TFs that play diverse roles in stem cell behavior and tissue specification. Analysis of single-cell RNA-sequencing (scRNA-seq) datasets identify a specific enrichment of Sox11 mRNA in differentiating but not quiescent MuSCs. Consistent with the scRNA-seq data, Sox11 levels increase during differentiation of murine primary myoblasts in vitro. scRNA-seq data comparing muscle regeneration in young and old mice further demonstrate that Sox11 expression is reduced in aged MuSCs. Age-related decline of Sox11 expression is associated with reduced chromatin contacts within the topologically associating domains. Unexpectedly, Myod1 Cre -driven deletion of Sox11 in embryonic myoblasts has no effects on muscle development and growth, resulting in apparently healthy muscles that regenerate normally. Pax7 CreER - or Rosa26 CreER - driven (MuSC-specific or global) deletion of Sox11 in adult mice similarly has no effects on MuSC differentiation or muscle regeneration. These results identify Sox11 as a novel myogenic differentiation marker with reduced expression in quiescent and aged MuSCs, but the specific function of Sox11 in myogenesis remains to be elucidated.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
Databáze: MEDLINE
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