Molecular detection of pathogens from ticks collected from dogs and cats at veterinary clinics in Finland.

Autor: Zakham F; Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.; Faculty of Pharmacy, University of Helsinki, Helsinki, Finland., Korhonen EM; Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland., Puonti PT; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland., Castrén RS; Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland., Uusitalo R; Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.; Department of Geosciences and Geography, University of Helsinki, Helsinki, Finland., Smura T; Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Clinical Microbiology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland., Kant R; Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland., Vapalahti O; Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.; Clinical Microbiology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland., Sironen T; Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland.; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland., Kinnunen PM; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland. Paula.Kinnunen@merck.com.; Companion Animal Business Unit, Nordic Cluster, MSD Animal Health, Espoo, Finland. Paula.Kinnunen@merck.com.
Jazyk: angličtina
Zdroj: Parasites & vectors [Parasit Vectors] 2023 Sep 13; Vol. 16 (1), pp. 327. Date of Electronic Publication: 2023 Sep 13.
DOI: 10.1186/s13071-023-05864-4
Abstrakt: Background: Ticks carry microbes, some of which are pathogenic for humans and animals. To assess this One Health challenge, 342 ticks were collected from pet dogs and cats at 10 veterinary clinics in Finland as part of the European project "Protect Our Future Too".
Methods: The tick species were identified, and ticks were screened with quantitative PCR (qPCR) for tick-borne pathogens, including Borrelia burgdorferi sensu lato, Borrelia miyamotoi, Ehrlichia canis, Anaplasma spp., Candidatus Neoehrlichia mikurensis, tick-borne encephalitis virus (TBEV), and Babesia spp. For comparison, a subset of tick DNA (20 qPCR-positive samples) was analysed with 16S next-generation sequencing (NGS).
Results: Most ticks were Ixodes ricinus (289, 84.5%), followed by Ixodes persulcatus (51, 14.9%). One hybrid tick (I. ricinus/I. persulcatus, 0.3%) and one Rhipicephalus sanguineus tick (0.3%) were identified. We found one or more of the analysed pathogens in 17% (59/342) of the ticks. The most prevalent pathogen was B. burgdorferi s.l. (36, 10.5%), followed by Anaplasma phagocytophilum (12, 3.5%), B. miyamotoi (5, 1.5%), Babesia venatorum (4, 1.2%), and TBEV (1, 0.3%). Candidatus Neoehrlichia mikurensis DNA was amplified from three (0.9%) ticks. Ehrlichia canis was not detected. In the 16S NGS, six samples produced enough reads for the analysis. In these six samples, we confirmed all the positive qPCR findings of Borrelia spp. and Ca. N. mikurensis.
Conclusions: The high prevalence of pathogenic microorganisms in the ticks of this study emphasizes the importance of awareness of ticks and tick-borne diseases and prevention. Furthermore, the results show that veterinary surveillance can facilitate early detection of tick-borne pathogens and new tick species and draw attention to possible co-infections that should be considered both in symptomatic humans and animals after tick bites.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
Databáze: MEDLINE
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