The tectonic complex regulates membrane protein composition in the photoreceptor cilium.
Autor: | Truong HM; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA., Cruz-Colón KO; Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI, USA., Martínez-Márquez JY; Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, USA., Willer JR; Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, USA., Travis AM; Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, USA., Biswas SK; Department of Neurobiology, Morehouse School of Medicine, Atlanta, GA, USA., Lo WK; Department of Neurobiology, Morehouse School of Medicine, Atlanta, GA, USA., Bolz HJ; Senckenberg Centre for Human Genetics, Frankfurt am Main, Germany.; Institute of Human Genetics, University Hospital of Cologne, Cologne, Germany., Pearring JN; Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, USA. pearring@umich.edu.; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA. pearring@umich.edu. |
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Jazyk: | angličtina |
Zdroj: | Nature communications [Nat Commun] 2023 Sep 13; Vol. 14 (1), pp. 5671. Date of Electronic Publication: 2023 Sep 13. |
DOI: | 10.1038/s41467-023-41450-z |
Abstrakt: | The primary cilium is a signaling organelle with a unique membrane composition maintained by a diffusional barrier residing at the transition zone. Many transition zone proteins, such as the tectonic complex, are linked to preserving ciliary composition but the mechanism remains unknown. To understand tectonic's role, we generate a photoreceptor-specific Tctn1 knockout mouse. Loss of Tctn1 results in the absence of the entire tectonic complex and associated MKS proteins yet has minimal effects on the transition zone structure of rod photoreceptors. We find that the protein composition of the photoreceptor cilium is disrupted as non-resident membrane proteins accumulate in the cilium over time, ultimately resulting in photoreceptor degeneration. We further show that fluorescent rhodopsin moves faster through the transition zone in photoreceptors lacking tectonic, which suggests that the tectonic complex acts as a physical barrier to slow down membrane protein diffusion in the photoreceptor transition zone to ensure proper removal of non-resident membrane proteins. (© 2023. Springer Nature Limited.) |
Databáze: | MEDLINE |
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