Single-cell isotope tracing reveals functional guilds of bacteria associated with the diatom Phaeodactylum tricornutum.

Autor: Mayali X; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA. mayali1@llnl.gov., Samo TJ; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Kimbrel JA; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Morris MM; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Rolison K; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Swink C; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Ramon C; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Kim YM; Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Munoz-Munoz N; Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Nicora C; Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Purvine S; Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Lipton M; Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA., Stuart RK; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Weber PK; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Sep 13; Vol. 14 (1), pp. 5642. Date of Electronic Publication: 2023 Sep 13.
DOI: 10.1038/s41467-023-41179-9
Abstrakt: Bacterial remineralization of algal organic matter fuels algal growth but is rarely quantified. Consequently, we cannot currently predict whether some bacterial taxa may provide more remineralized nutrients to algae than others. Here, we quantified bacterial incorporation of algal-derived complex dissolved organic carbon and nitrogen and algal incorporation of remineralized carbon and nitrogen in fifteen bacterial co-cultures growing with the diatom Phaeodactylum tricornutum at the single-cell level using isotope tracing and nanoSIMS. We found unexpected strain-to-strain and cell-to-cell variability in net carbon and nitrogen incorporation, including non-ubiquitous complex organic nitrogen utilization and remineralization. We used these data to identify three distinct functional guilds of metabolic interactions, which we termed macromolecule remineralizers, macromolecule users, and small-molecule users, the latter exhibiting efficient growth under low carbon availability. The functional guilds were not linked to phylogeny and could not be elucidated strictly from metabolic capacity as predicted by comparative genomics, highlighting the need for direct activity-based measurements in ecological studies of microbial metabolic interactions.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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