The extent of nitrogen isotopic fractionation in rumen bacteria is associated with changes in rumen nitrogen metabolism.

Autor: Cantalapiedra-Hijar G; INRAE, Université Clermont Auvergne, Vetagro Sup, UMRH, Saint-Genes-Champanelle, France., Martinez-Fernandez G; Agriculture and Food, CSIRO, St Lucia, QLD, Australia., Forano E; INRAE, Université Clermont Auvergne, UMR 454 MEDIS, Saint-Genès-Champanelle, France., Denman SE; Agriculture and Food, CSIRO, St Lucia, QLD, Australia., Morgavi D; INRAE, Université Clermont Auvergne, Vetagro Sup, UMRH, Saint-Genes-Champanelle, France., McSweeney CS; Agriculture and Food, CSIRO, St Lucia, QLD, Australia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Sep 13; Vol. 18 (9), pp. e0291243. Date of Electronic Publication: 2023 Sep 13 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0291243
Abstrakt: Nitrogen use efficiency is an important index in ruminants and can be indirectly evaluated through the N isotopic discrimination between the animal and its diet (Δ15Nanimal-diet). The concentration and source of N may determine both the extent of the N isotopic discrimination in bacteria and N use efficiency. We hypothesised that the uptake and release of ammonia by rumen bacteria will affect the natural 15N enrichment of the bacterial biomass over their substrates (Δ15Nbacteria-substrate) and thereby further impacting Δ15Nanimal-diet. To test this hypothesis, two independent in vitro experiments were conducted using two contrasting N sources (organic vs inorganic) at different levels either in pure rumen bacteria culture incubations (Experiment #1) or in mixed rumen cultures (Experiment #2). In Experiment #1, tryptone casein or ammonium chloride were tested at low (1 mM N) and high (11.5 mM N) concentrations on three rumen bacterial strains (Fibrobacter succinogenes, Eubacterium limosum and Xylanibacter ruminicola) incubated in triplicate in anaerobic batch monocultures during 48h. In Experiment #2 mixed rumen cultures were incubated during 120 h with peptone or ammonium chloride at five different levels of N (1.5, 3, 4.5, 6 and 12-mM). In experiment #1, Δ15Nbacteria-substrate was lowest when the ammonia-consumer bacterium Fibrobacter succinogenes was grown on ammonium chloride, and highest when the proteolytic bacterial strain Xylanibacter ruminicola was grown on tryptone. In experiment #2, Δ15Nbacteria-substrate was lower with inorganic (ammonium chloride) vs organic (peptone) N source. A strong negative correlation between Δ15Nbacteria-substrate and Rikenellaceae_RC9_gut_group, a potential fibrolytic rumen bacterium, was detected. Together, our results showed that Δ15Nbacteria-substrate may change according to the balance between synthesis of microbial protein from ammonia versus non-ammonia N sources and confirm the key role of rumen bacteria as modulators of Δ15Nanimal-diet.
Competing Interests: The authors declare no conflict of interest.
(Copyright: © 2023 Cantalapiedra-Hijar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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