In Vitro Evaluation of Rose Bengal Photoactivated by Custom-Built Green Light-Emitting Diode Source for Bacteria and Rapidly Growing Mycobacteria Inhibition.

Autor:	Trevizani Rocchetti T; Department of Ophthalmology and Visual Sciences, Escola Paulista de Medicina-Universidade Federal de São Paulo, São Paulo, Brazil., Alves Mendonça W Jr; Department of Ophthalmology and Visual Sciences, Escola Paulista de Medicina-Universidade Federal de São Paulo, São Paulo, Brazil., Caiado de Castro Neto J; Institute of Physics of São Carlos, Universidade de São Paulo, São Carlos, Brazil., Orlandi de Oliveira L; Institute of Physics of São Carlos, Universidade de São Paulo, São Carlos, Brazil., Orlandi de Oliveira A; Institute of Physics of São Carlos, Universidade de São Paulo, São Carlos, Brazil., de Freitas D; Department of Ophthalmology and Visual Sciences, Escola Paulista de Medicina-Universidade Federal de São Paulo, São Paulo, Brazil., Höfling-Lima AL; Department of Ophthalmology and Visual Sciences, Escola Paulista de Medicina-Universidade Federal de São Paulo, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Translational vision science & technology [Transl Vis Sci Technol] 2023 Sep 01; Vol. 12 (9), pp. 9.
DOI:	10.1167/tvst.12.9.9
Abstrakt:	Purpose: In vitro evaluation of rose bengal (RB) photoactivated by our custom-built green light-emitting diode (LED) source for the growth inhibition of bacterial strains and rapidly growing mycobacterial (RGM) isolates in infectious keratitis. Methods: Six corneal clinical bacteria isolates were included in this study: two Gram-positive bacteria (methicillin-resistant Staphylococcus aureus [MRSA] and Staphylococcus epidermidis), two Gram-negative bacteria (Pseudomonas aeruginosa and Serratia marcescens), and two RGM (Mycobacterium chelonae and Mycobacterium abscessus). Microorganisms were cultured and incubated at specific conditions and prepared in suspensions to adjust their concentration to 104 cells/mL. Different treatments were conducted in triplicates: Group I, no treatment; Group II, treated with 0.1% rose bengal alone (exposed to dark for 30 minutes); Group III, exposed to custom green LED for 30 minutes (12.87 J/cm2); and Group IV, treated with 0.1% rose bengal and exposed to custom green LED for 30 minutes. Agar plates were incubated at specific conditions and photographed after growth for pixel analyses. Results: Complete growth inhibition of all bacteria and RGM was observed in Group IV. MRSA and S. epidermidis in Group II also showed complete growth inhibition. Conclusions: The custom-built green LED presented good activity by photoactivating RB and inhibiting micro-organism growth. For the first time, we demonstrated the expressive growth inhibition effect of RB against S. epidermidis, RGM, and S. marcescens. Clinical treatment with RB may offer an alternate adjunct therapy for corneal surface infections. Translational Relevance: Validating in vitro the custom-built green LED encourages the clinical application for the treatment of infectious keratitis.
Databáze:	MEDLINE
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