Oxidative stress associated with proteomic and fatty acid profiles of sperm from Nellore bulls at rest†.

Autor:	Ramírez-López CJ; Animal Reproduction Laboratory, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.; Structural Biology Laboratory, Department of Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerai, Brazil., Barros E; Nucleus for Analysis of Biomolecules, Universidade Federal de Viçosa, Brazil., Vidigal PM; Nucleus for Analysis of Biomolecules, Universidade Federal de Viçosa, Brazil., Okano DS; Animal Reproduction Laboratory, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Gomes LL; Animal Reproduction Laboratory, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Carvalho RPR; Structural Biology Laboratory, Department of Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerai, Brazil., de Castro AG; Biotechnology and Biodiversity for the Environment Laboratory, Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Baracat-Pereira MC; Proteomics and Protein Biochemistry Laboratory, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Guimarães SEF; LABTEC-Animal Biotechnology Laboratory, Department of Animal Science, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil., Guimarães JD; Animal Reproduction Laboratory, Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2023 Dec 11; Vol. 109 (6), pp. 878-891.
DOI:	10.1093/biolre/ioad121
Abstrakt:	Sexual rest is a transient condition, which compromises conception rates, characterized by large volumes of ejaculate with high percentages of dead sperm observed in bulls. The biochemical mechanisms leading to this ejaculate pattern are not fully understood. Six adult resting Nellore bulls were submitted to Breeding Soundness Evaluation by four consecutive semen collections through the electroejaculation method during a 30 min period. Each ejaculate had its semen phenotypic parameters; morphology and physical aspects were evaluated. To assess enzymatic activity (superoxide dismutase, catalase, and glutathione S-transferase), lipid peroxidation (concentrations of malondialdehyde and nitric oxide), fatty acid, and proteomic profile aliquots of spermatozoa from the first and fourth ejaculates were used. All sperm parameters differed between the first and fourth ejaculates. Spermatozoa from the first ejaculate showed lower enzymatic activity and a higher concentration of lipid peroxidation markers. Among the 19 identified fatty acids, 52.7% are polyunsaturated. Relative abundance analysis showed that C12:0 and C18:0 fatty acids differed between the first and fourth ejaculates, being the fourth ejaculate richer in spermatozoa. The proteomics analysis identified a total of 974 proteins in both sample groups (first and fourth ejaculates). The majority of identified proteins are related to cellular processes and signaling. Quantitative proteomics showed 36 differentially abundant proteins, 6 up-regulated proteins in the first ejaculate, and 30 up-regulated proteins in the fourth ejaculate. Spermatozoa from bulls at sexual rest have less antioxidant capacity, causing changes in their fatty acid composition and protein profile, which generates the observed sperm pattern and lower fertilization capacity. (© The Author(s) 2023. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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