Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model.

Autor: Munir A; Department of Biomaterials Institute of Clinical Dentistry, University of Oslo Oslo Norway., Reseland JE; Department of Biomaterials Institute of Clinical Dentistry, University of Oslo Oslo Norway., Tiainen H; Department of Biomaterials Institute of Clinical Dentistry, University of Oslo Oslo Norway., Haugen HJ; Department of Biomaterials Institute of Clinical Dentistry, University of Oslo Oslo Norway., Sikorski P; Department of Physics Norwegian University of Science and Technology (NTNU) Trondheim Norway., Christiansen EF; Department of Physics Norwegian University of Science and Technology (NTNU) Trondheim Norway., Reinholt FP; Department of Pathology Oslo University Hospital Oslo Norway., Syversen U; Department of Clinical and Molecular Medicine Norwegian University of Science and Technology Trondheim Norway., Solberg LB; Department of Pathology Oslo University Hospital Oslo Norway.; Division of Orthopedic Surgery Oslo University Hospital Oslo Norway.
Jazyk: angličtina
Zdroj: JBMR plus [JBMR Plus] 2023 Jun 28; Vol. 7 (9), pp. e10792. Date of Electronic Publication: 2023 Jun 28 (Print Publication: 2023).
DOI: 10.1002/jbm4.10792
Abstrakt: In vitro models of primary human osteocytes embedded in natural mineralized matrix without artificial scaffolds are lacking. We have established cell culture conditions that favored the natural 3D orientation of the bone cells and stimulated the cascade of signaling needed for primary human osteoblasts to differentiate into osteocytes with the characteristically phenotypical dendritic network between cells. Primary human osteoblasts cultured in a 3D rotating bioreactor and incubated with a combination of vitamins A, C, and D for up to 21 days produced osteospheres resembling native bone. Osteocyte-like cells were identified as entrapped, stellate-shaped cells interconnected through canaliculi embedded in a structured, mineralized, collagen matrix. These cells expressed late osteoblast and osteocyte markers such as osteocalcin (OCN), podoplanin (E11), dentin matrix acidic phosphoprotein 1 (DMP1), and sclerostin (SOST). Organized collagen fibrils, observed associated with the cell hydroxyapatite (HAp) crystals, were found throughout the spheroid and in between the collagen fibrils. In addition to osteocyte-like cells, the spheroids consisted of osteoblasts at various differentiation stages surrounded by a rim of cells resembling lining cells. This resemblance to native bone indicates a model system with potential for studying osteocyte-like cell differentiation, cross-talk between bone cells, and the mineralization process in a bonelike structure in vitro without artificial scaffolds. In addition, natural extracellular matrix may allow for the study of tissue-specific biochemical, biophysical, and mechanical properties. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
(© 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.)
Databáze: MEDLINE
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