Copper bioreduction and nanoparticle synthesis by an enrichment culture from a former copper mine.

Autor: Kimber RL; Department of Earth and Environmental Sciences, Williamson Research Centre for Molecular Environmental Science, School of Natural Sciences, University of Manchester, Manchester, UK.; Department of Environmental Geosciences, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria., Elizondo G; Department of Earth and Environmental Sciences, Williamson Research Centre for Molecular Environmental Science, School of Natural Sciences, University of Manchester, Manchester, UK., Jedyka K; Department of Earth and Environmental Sciences, Williamson Research Centre for Molecular Environmental Science, School of Natural Sciences, University of Manchester, Manchester, UK., Boothman C; Department of Earth and Environmental Sciences, Williamson Research Centre for Molecular Environmental Science, School of Natural Sciences, University of Manchester, Manchester, UK., Cai R; Department of Materials, University of Manchester, Manchester, UK., Bagshaw H; SEM Shared Research Facility, School of Engineering, University of Liverpool, Liverpool, UK., Haigh SJ; Department of Materials, University of Manchester, Manchester, UK., Coker VS; Department of Earth and Environmental Sciences, Williamson Research Centre for Molecular Environmental Science, School of Natural Sciences, University of Manchester, Manchester, UK., Lloyd JR; Department of Earth and Environmental Sciences, Williamson Research Centre for Molecular Environmental Science, School of Natural Sciences, University of Manchester, Manchester, UK.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2023 Dec; Vol. 25 (12), pp. 3139-3150. Date of Electronic Publication: 2023 Sep 11.
DOI: 10.1111/1462-2920.16488
Abstrakt: Microorganisms can facilitate the reduction of Cu 2+ , altering its speciation and mobility in environmental systems and producing Cu-based nanoparticles with useful catalytic properties. However, only a few model organisms have been studied in relation to Cu 2+ bioreduction and little work has been carried out on microbes from Cu-contaminated environments. This study aimed to enrich for Cu-resistant microbes from a Cu-contaminated soil and explore their potential to facilitate Cu 2+ reduction and biomineralisation from solution. We show that an enrichment grown in a Cu-amended medium, dominated by species closely related to Geothrix fermentans, Azospira restricta and Cellulomonas oligotrophica, can reduce Cu 2+ with subsequent precipitation of Cu nanoparticles. Characterisation of the nanoparticles with (scanning) transmission electron microscopy, energy-dispersive x-ray spectroscopy and electron energy loss spectroscopy supports the presence of both metallic Cu(0) and S-rich Cu(I) nanoparticles. This study provides new insights into the diversity of microorganisms capable of facilitating copper reduction and highlights the potential for the formation of distinct nanoparticle phases resulting from bioreduction or biomineralisation reactions. The implications of these findings for the biogeochemical cycling of copper and the potential biotechnological synthesis of commercially useful copper nanoparticles are discussed.
(© 2023 The Authors. Environmental Microbiology published by Applied Microbiology International and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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