Natural Products Targeting the Fungal Unfolded Protein Response as an Alternative Crop Protection Strategy.

Autor:	Charpentier T; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France.; Université Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, 49070 Beaucouzé, France., Viault G; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France., Le Ray AM; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France., Bataillé-Simoneau N; Université Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, 49070 Beaucouzé, France., Helesbeux JJ; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France., Blon N; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France., Bastide F; Université Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, 49070 Beaucouzé, France., Marchi M; Université Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, 49070 Beaucouzé, France., Aligon S; Université Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, 49070 Beaucouzé, France., Bruguière A; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France., Dinh CP; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France., Benbelkacem Z; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France., Dallery JF; Université Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, 49070 Beaucouzé, France., Simoneau P; Université Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, 49070 Beaucouzé, France., Richomme P; Université Angers, SONAS, SFR QUASAV, F-49000 Angers, France., Guillemette T; Université Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, 49070 Beaucouzé, France.
Jazyk:	angličtina
Zdroj:	Journal of agricultural and food chemistry [J Agric Food Chem] 2023 Sep 20; Vol. 71 (37), pp. 13706-13716. Date of Electronic Publication: 2023 Sep 11.
DOI:	10.1021/acs.jafc.3c03602
Abstrakt:	Discovering new solutions for crop protection is a major challenge for the next decades as a result of the ecotoxicological impact of classical fungicides, the emergence of fungicide resistances, and the consequence of climate change on pathogen distribution. Previous work on fungal mutants deficient in the unfolded protein response (UPR) supported that targeting this pathway is a promising plant disease control strategy. In particular, we showed that the UPR is involved in fungal virulence by altering cell protection against host defense compounds, such as phytoalexins and phytoanticipins. In this study, we evaluated natural products targeting fungal IRE1 protein (UPR effector) and consequently increasing fungal susceptibility to plant defenses. Developing an in vitro cell-based screening assay allowed for the identification of seven potential IRE1 inhibitors with a focus on polyhydroxylated prenylated xanthones. Inhibition of hac 1 mRNA splicing, which is mediated by IRE1, was then validated for the most active compound, namely, γ-mangostin 3 . To study the mode of interaction between the binding site of IRE1 and active xanthones, molecular docking was also undertaken, revealing similar and novel interactions between the known inhibitor and the binding site. Eventually, active xanthones applied at subtoxic doses induced a significant reduction in necrosis size for leaves of Brassica oleracea inoculated with Alternaria brassicicola and Botrytis cinerea .
Databáze:	MEDLINE
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