Modified Neuropixels probes for recording human neurophysiology in the operating room.

Autor:	Coughlin B; Center for Neurotechnology and Neurorecovery, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.; Department of Neurology, Harvard Medical School, Boston, MA, USA., Muñoz W; Department of Neurosurgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA., Kfir Y; Department of Neurosurgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA., Young MJ; Center for Neurotechnology and Neurorecovery, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.; Department of Neurology, Harvard Medical School, Boston, MA, USA., Meszéna D; Center for Neurotechnology and Neurorecovery, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.; Department of Neurology, Harvard Medical School, Boston, MA, USA., Jamali M; Department of Neurosurgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA., Caprara I; Department of Neurosurgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA., Hardstone R; Center for Neurotechnology and Neurorecovery, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.; Department of Neurology, Harvard Medical School, Boston, MA, USA., Khanna A; Department of Neurosurgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA., Mustroph ML; Department of Neurosurgery, Harvard Medical School and Brigham & Women's Hospital, Boston, MA, USA., Trautmann EM; Department of Neuroscience, Columbia University Medical Center, New York, NY, USA.; Zuckerman Institute, Columbia University, New York, NY, USA.; Grossman Center for the Statistics of Mind, Columbia University Medical Center, New York, NY, USA., Windolf C; Department of Statistics, Zuckerman Institute, Columbia University, New York, NY, USA., Varol E; Department of Statistics, Zuckerman Institute, Columbia University, New York, NY, USA.; Department of Computer Science and Engineering, Zuckerman Institute, Columbia University, New York, NY, USA., Soper DJ; Center for Neurotechnology and Neurorecovery, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.; Department of Neurology, Harvard Medical School, Boston, MA, USA., Stavisky SD; Department of Neurological Surgery, University of California Davis, Davis, CA, USA.; Department of Neurosurgery, Stanford University, Stanford, CA, USA.; Wu Tsai Neurosciences Institute and Bio-X Institute, Stanford University, Stanford, CA, USA.; Howard Hughes Medical Institute at Stanford University, Stanford, CA, USA., Welkenhuysen M; imec, Leuven, Belgium., Dutta B; imec, Leuven, Belgium., Shenoy KV; Department of Neurosurgery, Stanford University, Stanford, CA, USA.; Wu Tsai Neurosciences Institute and Bio-X Institute, Stanford University, Stanford, CA, USA.; Howard Hughes Medical Institute at Stanford University, Stanford, CA, USA.; Department of Electrical Engineering, Stanford University, Stanford, CA, USA.; Department of Bioengineering, Stanford University, Stanford, CA, USA.; Department of Neurobiology, Stanford University, Stanford, CA, USA., Hochberg LR; Center for Neurotechnology and Neurorecovery, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA.; Department of Neurology, Harvard Medical School, Boston, MA, USA.; VA RR&D Center for Neurorestoration and Neurotechnology, Rehabilitation R&D Service, Providence VA Medical Center, Providence, RI, USA.; School of Engineering and Carney Institute for Brain Science, Brown University, Providence, RI, USA., Mark Richardson R; Department of Neurosurgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA., Williams ZM; Department of Neurosurgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA. zwilliams@mgh.harvard.edu., Cash SS; Center for Neurotechnology and Neurorecovery, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA. cash@mgh.harvard.edu.; Department of Neurology, Harvard Medical School, Boston, MA, USA. cash@mgh.harvard.edu., Paulk AC; Center for Neurotechnology and Neurorecovery, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA. apaulk@mgh.harvard.edu.; Department of Neurology, Harvard Medical School, Boston, MA, USA. apaulk@mgh.harvard.edu.
Jazyk:	angličtina
Zdroj:	Nature protocols [Nat Protoc] 2023 Oct; Vol. 18 (10), pp. 2927-2953. Date of Electronic Publication: 2023 Sep 11.
DOI:	10.1038/s41596-023-00871-2
Abstrakt:	Neuropixels are silicon-based electrophysiology-recording probes with high channel count and recording-site density. These probes offer a turnkey platform for measuring neural activity with single-cell resolution and at a scale that is beyond the capabilities of current clinically approved devices. Our team demonstrated the first-in-human use of these probes during resection surgery for epilepsy or tumors and deep brain stimulation electrode placement in patients with Parkinson's disease. Here, we provide a better understanding of the capabilities and challenges of using Neuropixels as a research tool to study human neurophysiology, with the hope that this information may inform future efforts toward regulatory approval of Neuropixels probes as research devices. In perioperative procedures, the major concerns are the initial sterility of the device, maintaining a sterile field during surgery, having multiple referencing and grounding schemes available to de-noise recordings (if necessary), protecting the silicon probe from accidental contact before insertion and obtaining high-quality action potential and local field potential recordings. The research team ensures that the device is fully operational while coordinating with the surgical team to remove sources of electrical noise that could otherwise substantially affect the signals recorded by the sensitive hardware. Prior preparation using the equipment and training in human clinical research and working in operating rooms maximize effective communication within and between the teams, ensuring high recording quality and minimizing the time added to the surgery. The perioperative procedure requires ~4 h, and the entire protocol requires multiple weeks. (© 2023. Springer Nature Limited.)
Databáze:	MEDLINE
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