Autor: |
Wei PY; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, China.; Shaanxi Key Laboratory of Plant Nematology, Xi'an 710043, China., Pan S; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, China.; Shaanxi Key Laboratory of Plant Nematology, Xi'an 710043, China., Peng DL; Shaanxi Key Laboratory of Plant Nematology, Xi'an 710043, China.; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Zhang F; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, China.; Shaanxi Key Laboratory of Plant Nematology, Xi'an 710043, China., Chen ZJ; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, China.; Shaanxi Key Laboratory of Plant Nematology, Xi'an 710043, China., Zhang SL; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, China.; Shaanxi Key Laboratory of Plant Nematology, Xi'an 710043, China., Li YM; Bio-Agriculture Institute of Shaanxi, Xi'an 710043, China.; Shaanxi Key Laboratory of Plant Nematology, Xi'an 710043, China. |
Abstrakt: |
We examined the effects of low temperature on egg hatching and killing rate of the 2nd instars of Meloi-dogyne incognita (J2) in the laboratory. We further evaluated the effects of two soil treatment methods on the survival rate of M. incognita in northern China in a field experiment. The results of laboratory experiment showed that survival rate of J2 was 0 after being subjected to -7 ℃ for 24 hours, and that egg hatching was completely inhibited 24 hours after being subjected to -9 ℃. The survival rate of J2 was 0 after being subjected to -1, -2, -3, and -4 ℃ for 8, 5, 3, and 1.5 d, respectively. Egg hatching was completely inhibited after being subjected to -2, -3, -4, and -5 ℃ for 9, 6, 4, and 1 d, respectively. Results of the fitting analysis showed that both the relationships between the temperature and the lethal time of J2 as well as the temperature and the non-hatching time of the eggs followed exponential functions. The results of field test showed that death rate of M. incognita in 0-50 cm soil layer after ridging treatment and 0-30 cm soil layer after leveling treatment could reach 100%, while the disease index of the former in 30-40 cm and 40-50 cm was 84.9% and 75.8%, respectively, which was lower than that in the greenhouse. Our results suggest that preventing and controlling M. incognita in greenhouses through low-tempe-rature in winter could achieve a better control effect in Yulin City and the northward region. The proposed technique is convenient and has high potential for popularization. |