| Autor: |
Koirala P; Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045 USA., Doody C; Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706 USA., Blackwell H; Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706 USA., Chandler JR; Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045 USA. |
| Jazyk: |
angličtina |
| Zdroj: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Sep 03. Date of Electronic Publication: 2023 Sep 03. |
| DOI: |
10.1101/2023.09.02.556004 |
| Abstrakt: |
The soil bacterium Chromobacterium substugae uses a single LuxI-R-type quorum-sensing system, CviI-R, to regulate genes in a cell density-dependent manner. CviI synthesizes the signal N -hexanoyl-homoserine lactone (C6-HSL) and CviR is a C6-HSL-responsive cytoplasmic transcription regulator. C6-HSL-bound CviR activates dozens of genes, for example the cdeAB-oprM cluster coding for an efflux pump conferring antibiotic resistance. The cdeAB-oprM genes are also regulated by an antibiotic-responsive transcription factor, CdeR, which represses expression of these genes. We are interested in understanding how C. subtsugae integrates different environmental cues to regulate antibiotic resistance. In this study, we sought to delineate the mechanism of regulation of the cdeAB-oprM genes by CviR and CdeR. In recombinant E. coli, the cdeA promoter is activated by CviR and repressed by CdeR. We identify non-overlapping sequence elements in the cdeA promoter that are required for CviR activation and CdeR repression, respectively. We also examined the role of CdeR in modulating cdeA activation by C6-HSL in C. subtsugae . We show that CviR and CdeR can independently modulate transcription from the cdeA promoter in C. subtsugae , consistent with the conclusion that CviR and CdeR regulate the cdeAB-oprM genes by interacting directly with different binding sites in the cdeA promoter. These results contribute to a molecular understanding of how the cdeAB-oprM genes are regulated and provide new insight into how C. subtsugae integrates different environmental cues to regulate antibiotic resistance. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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