Bioinformatics, RNA sequencing, and targeted bisulfite sequencing analyses identify the role of PROM2 as a diagnostic and prognostic biomarker.

Autor:	Uqaili AA; Department of Physiology, Liaquat University of Medical and Health Sciences Jamshoro 76090, Pakistan., Usman G; Department of Community Medicine, LUMHS Jamshoro 76090, Pakistan., Bhatti U; Department of Physiology, LUMHS Jamshoro 76090, Pakistan., Nasir H; Clinical and Translational Oncology, Scuola Superiore Meridionale, Naples Federico II University Naples 80131, Italy., Zia R; Pakistan Kidney and Liver Institute and Research Center Lahore 54000, Pakistan., Akram MA; Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University Rawalpindi 46000, Pakistan., Jawad FA; Centre of Agricultural Biochemistry and Biotechnology-University of Agriculture Faisalabad Faisalabad 38040, Pakistan., Farid A; Department of Botany, PMAS Arid Agriculture University Rawalpindi 46000, Pakistan., AbdelGawwad MR; Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, International University of Sarajevo Sarajevo 71210, Bosnia and Herzegovina., Almutairi SM; Department of Botany and Microbiology, College of Science, King Saud University P.O. 2455, Riyadh 11451, Saudi Arabia., Elshikh MS; Department of Botany and Microbiology, College of Science, King Saud University P.O. 2455, Riyadh 11451, Saudi Arabia., Hussain S; Department of Botany, PMAS Arid Agriculture University Rawalpindi 46000, Pakistan., Rasheed RA; Histology & Cell Biology Department, Faculty of Medicine, King Salman International University South Sinai, Egypt.
Jazyk:	angličtina
Zdroj:	American journal of translational research [Am J Transl Res] 2023 Aug 15; Vol. 15 (8), pp. 5389-5407. Date of Electronic Publication: 2023 Aug 15 (Print Publication: 2023).
Abstrakt:	Objectives: Prominin 2 (PROM2) gene has been reported as a molecular biomarker of human cancers; however, its role is still controversial. This study was therefore arranged to seek the role of PROM2 in different cancers with Bioinformatics and in vitro analyses. Methods: A combination of bioinformatics and molecular experiments. Results: Through the utilization of Bioinformatics analysis, it was observed that in 19 out of the 24 human cancers studied, there was a significant increase in the expression of PROM2 compared to the respective control samples. Additionally, the overexpression of PROM2 was linked specifically to a decrease in overall survival (OS) among breast cancer (BRCA), lung adenocarcinoma (LUAD), and uterine corpus endometrial carcinoma (UCEC) patients. Furthermore, advanced molecular investigations were conducted, encompassing RNA sequencing (RNA-seq) as well as targeted bisulfite sequencing (bisulfite-seq) assessments of PROM2. These analyses were performed across an array of lung cancer cell lines (A549, ABC-1, EBC-1, and LK-2) and a normal control lung cell line (MRC-9). Results of these analysis revealed overexpression and reduced methylation of PROM2 within lung cancer cell lines, relative to the corresponding control cell line. This suggests that PROM2 assumes a substantial function in the advancement and course of BRCA, LUAD, and UCEC cancers. Subsequent pathway analysis revealed that genes enriched by PROM2 are actively engaged in four pivotal pathways. Additionally, intriguing associations were observed between PROM2 expression, tumor purity, infiltration of CD8+ T immune cells, and genetic modifications. Moreover, we also predicted a few MicroRNAs (miRNAs), transcription factors (TFs), and potential drugs that could help to understand and better manage these cancers via designing appropriate therapies targeting PROM2. Conclusion: Via this study, we effectively revealed PROM2 overexpression as a potential diagnostic and prognostic biomarker of survival in BRCA, LUAD, and UCEC. Competing Interests: None. (AJTR Copyright © 2023.)
Databáze:	MEDLINE
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