Fine mapping and sequence analysis reveal a promising candidate gene encoding a novel NB-ARC domain derived from wild rice ( Oryza officinalis ) that confers bacterial blight resistance.

Autor:	Sinha P; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Kumar T D; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Sk H; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Solanki M; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Gokulan CG; Crop Improvement, Council of Scientific & Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad, India., Das A; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Miriyala A; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Gonuguntala R; Seeds/Biotech R&D Division, Rallis India Limited, Bangalore, India., Elumalai P; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., M B V N K; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., S K M; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Kumboju C; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Arra Y; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India.; Institute of Molecular Physiology, Heinrich Heine University, Düsseldorf, Germany., G S L; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Chirravuri NN; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Patel HK; Crop Improvement, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India., Ghazi IA; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India., Kim SR; Rice Breeding Innovation Platform, International Rice Research Institute (IRRI), Los Banos, Philippines., Jena KK; School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT) University, Bhubaneswar, Odisha, India., Hanumanth SR; Department of Genetics, Osmania University, Hyderabad, India., Oliva R; Safe and Sustainable Value Chain, World Vegetable Center, Tainan, Taiwan., Mangrauthia SK; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India., Sundaram RM; Department of Biotechnology, ICAR-Indian Institute of Rice Research, Hyderabad, India.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Aug 25; Vol. 14, pp. 1173063. Date of Electronic Publication: 2023 Aug 25 (Print Publication: 2023).
DOI:	10.3389/fpls.2023.1173063
Abstrakt:	Bacterial blight disease of rice caused by Xanthomonas oryzae pv. oryzae ( Xoo ) is one of the most serious constraints in rice production. The most sustainable strategy to combat the disease is the deployment of host plant resistance. Earlier, we identified an introgression line, IR 75084-15-3-B-B, derived from Oryza officinalis possessing broad-spectrum resistance against Xoo . In order to understand the inheritance of resistance in the O. officinalis accession and identify genomic region(s) associated with resistance, a recombinant inbred line (RIL) mapping population was developed from the cross Samba Mahsuri (susceptible to bacterial blight) × IR 75084-15-3-B-B (resistant to bacterial blight). The F 2 population derived from the cross segregated in a phenotypic ratio of 3: 1 (resistant susceptible) implying that resistance in IR 75084-15-3-B-B is controlled by a single dominant gene/quantitative trait locus (QTL). In the F 7 generation, a set of 47 homozygous resistant lines and 47 homozygous susceptible lines was used to study the association between phenotypic data obtained through screening with Xoo and genotypic data obtained through analysis of 7K rice single-nucleotide polymorphism (SNP) chip. Through composite interval mapping, a major locus was detected in the midst of two flanking SNP markers, viz., Chr11.27817978 and Chr11.27994133, on chromosome 11L with a logarithm of the odds (LOD) score of 10.21 and 35.93% of phenotypic variation, and the locus has been named Xa48t . In silico search in the genomic region between the two markers flanking Xa48t identified 10 putatively expressed genes located in the region of interest. The quantitative expression and DNA sequence analysis of these genes from contrasting parents identified the Os11g0687900 encoding an NB-ARC domain-containing protein as the most promising gene associated with resistance. Interestingly, a 16-bp insertion was noticed in the untranslated region (UTR) of the gene in the resistant parent, IR 75084-15-3-B-B, which was absent in Samba Mahsuri. The association of Os11g0687900 with resistance phenotype was further established by sequence-based DNA marker analysis in the RIL population. A co-segregating PCR-based INDEL marker, Marker_Xa48, has been developed for use in the marker-assisted breeding of Xa48t . Competing Interests: Author RG was employed by the company Rallis India Limited. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Sinha, Kumar T., Sk, Solanki, Gokulan, Das, Miriyala, Gonuguntala, Elumalai, M. B. V. N, S. K., Kumboju, Arra, G. S., Chirravuri, Patel, Ghazi, Kim, Jena, Hanumanth, Oliva, Mangrauthia and Sundaram.)
Databáze:	MEDLINE
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