Extracellular matrix type 0: From ancient collagen lineage to a versatile product pipeline - JellaGel™.

Autor:	Faruqui N; National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, UK., Williams DS; Jellagen Ltd, Capital Business Park, Cardiff, CF3 2PX, UK., Briones A; National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, UK., Kepiro IE; National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, UK., Ravi J; National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, UK., Kwan TOC; National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, UK., Mearns-Spragg A; Jellagen Ltd, Capital Business Park, Cardiff, CF3 2PX, UK., Ryadnov MG; National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, UK.
Jazyk:	angličtina
Zdroj:	Materials today. Bio [Mater Today Bio] 2023 Aug 29; Vol. 22, pp. 100786. Date of Electronic Publication: 2023 Aug 29 (Print Publication: 2023).
DOI:	10.1016/j.mtbio.2023.100786
Abstrakt:	Extracellular matrix type 0 is reported. The matrix is developed from a jellyfish collagen predating mammalian forms by over 0.5 billion years. With its ancient lineage, compositional simplicity, and resemblance to multiple collagen types, the matrix is referred to as the extracellular matrix type 0. Here we validate the matrix describing its physicochemical and biological properties and present it as a versatile, minimalist biomaterial underpinning a pipeline of commercialised products under the collective name of JellaGel TM . We describe an extensive body of evidence for folding and assembly of the matrix in comparison to mammalian matrices, such as bovine collagen, and its use to support cell growth and development in comparison to known tissue-derived products, such as Matrigel™. We apply the matrix to co-culture human astrocytes and cortical neurons derived from induced pluripotent stem cells and visualise neuron firing synchronicity with correlations indicative of a homogenous extracellular material in contrast to the performance of heterogenous commercial matrices. We prove the ability of the matrix to induce spheroid formation and support the 3D culture of human immortalised, primary, and mesenchymal stem cells. We conclude that the matrix offers an optimal solution for systemic evaluations of cell-matrix biology. It effectively combines the exploitable properties of mammalian tissue extracts or top-down matrices, such as biocompatibility, with the advantages of synthetic or bottom-up matrices, such as compositional control, while avoiding the drawbacks of the two types, such as biological and design heterogeneity, thereby providing a unique bridging capability of a stem extracellular matrix. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:. Andrew Mearns Spragg has patent #20230148082 pending to Jellagen Limited. (© 2023 The Authors.)
Databáze:	MEDLINE
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