Hormonal and proteomic analyses of southern blight disease caused by Athelia rolfsii and root chitosan priming on Cannabis sativa in an in vitro hydroponic system.

Autor: Suwanchaikasem P; School of BioSciences University of Melbourne Melbourne Victoria Australia., Nie S; Mass Spectrometry and Proteomics Facility, Bio21 Molecular Science and Biotechnology Institute University of Melbourne Melbourne Victoria Australia., Selby-Pham J; School of BioSciences University of Melbourne Melbourne Victoria Australia.; Cannabis and Biostimulants Research Group Pty Ltd Melbourne Victoria Australia., Walker R; School of BioSciences University of Melbourne Melbourne Victoria Australia., Boughton BA; School of BioSciences University of Melbourne Melbourne Victoria Australia.; Australian National Phenome Centre Murdoch University Perth Western Australia Australia., Idnurm A; School of BioSciences University of Melbourne Melbourne Victoria Australia.
Jazyk: angličtina
Zdroj: Plant direct [Plant Direct] 2023 Sep 08; Vol. 7 (9), pp. e528. Date of Electronic Publication: 2023 Sep 08 (Print Publication: 2023).
DOI: 10.1002/pld3.528
Abstrakt: Southern blight disease, caused by the fungal pathogen Athelia rolfsii , suppresses plant growth and reduces product yield in Cannabis sativa agriculture. Mechanisms of pathology of this soil-borne disease remain poorly understood, with disease management strategies reliant upon broad-spectrum antifungal use. Exposure to chitosan, a natural elicitor, has been proposed as an alternative method to control diverse fungal diseases in an eco-friendly manner. In this study, C. sativa plants were grown in the Root-TRAPR system, a transparent hydroponic growth device, where plant roots were primed with .2% colloidal chitosan prior to A. rolfsii inoculation. Both chitosan-primed and unprimed inoculated plants displayed classical symptoms of wilting and yellowish leaves, indicating successful infection. Non-primed infected plants showed increased shoot defense responses with doubling of peroxidase and chitinase activities. The levels of growth and defense hormones including auxin, cytokinin, and jasmonic acid were increased 2-5-fold. In chitosan-primed infected plants, shoot peroxidase activity and phytohormone levels were decreased 1.5-4-fold relative to the unprimed infected plants. When compared with shoots, roots were less impacted by A. rolfsii infection, but the pathogen secreted cell wall-degrading enzymes into the root-growth solution. Chitosan priming inhibited root growth, with root lengths of chitosan-primed plants approximately 65% shorter than the control, but activated root defense responses, with root peroxidase activity increased 2.7-fold along with increased secretion of defense proteins. The results suggest that chitosan could be an alternative platform to manage southern blight disease in C. sativa cultivation; however, further optimization is required to maximize effectiveness of chitosan.
Competing Interests: This work was partly financially supported by Nutrifield Pty Ltd, but the findings of this study are not used for any commercial purpose.
(© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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