CALU-3 lung cells three-dimensionally assembled onto CellFate® matrix present angiotensin-converting enzyme-2 activity.
Autor: | Dos Santos JF; Laboratory of Skin Physiology and Tissue Bioengineering, School of Arts, Sciences and Humanities (EACH) of University of Sao Paulo, Sao Paulo, Brazil., Dos Reis EM; Biocelltis Biotechnology SA, Florianópolis, Brazil., Berti FV; Biocelltis Biotechnology SA, Florianópolis, Brazil., Colla G; Biocelltis Biotechnology SA, Florianópolis, Brazil., Koepp J; Biocelltis Biotechnology SA, Florianópolis, Brazil., Nunes VA; Laboratory of Skin Physiology and Tissue Bioengineering, School of Arts, Sciences and Humanities (EACH) of University of Sao Paulo, Sao Paulo, Brazil. |
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Jazyk: | angličtina |
Zdroj: | Biotechnology and bioengineering [Biotechnol Bioeng] 2023 Dec; Vol. 120 (12), pp. 3602-3611. Date of Electronic Publication: 2023 Sep 10. |
DOI: | 10.1002/bit.28552 |
Abstrakt: | Currently, there is a great need for the development of three-dimensional (3D) in vitro lung models. Particularly, the production of a suitable 3D model of pulmonary epithelium for understanding the pathophysiology of diseases such as the COVID-19 must consider the tissue architecture and presence, for example, of the angiotensin-converting enzyme-2 (ACE-2) in the cells. Different polymeric membranes are being used to support cell culturing, especially of lung cells, however, there is still no information about the culture of these cells onto bacterial nanocellulose (BNC) matrices. We have used the BNC matrix CellFate® as a support for the assembly of a 3D in vitro model of lung epithelium, composed of human lung fibroblasts (HLF) and lung adenocarcinoma cells (CALU-3). CellFate® matrices were made from bacterial fermentation resulting in a natural and biocompatible biopolymer. Cells were cultured onto CellFate® and maintained in a 5% CO (© 2023 Wiley Periodicals LLC.) |
Databáze: | MEDLINE |
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