High-throughput screening and clinical importance of autophagy-associated genes in basal cell carcinoma.

Autor: Ulutas S; Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey. Electronic address: saffetulutas@gmail.com., Mutaf M; Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey., Koc MN; Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey., Oztuzcu T; Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey., Ulasli M; Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey., Oztuzcu S; Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
Jazyk: angličtina
Zdroj: Pathology, research and practice [Pathol Res Pract] 2023 Oct; Vol. 250, pp. 154786. Date of Electronic Publication: 2023 Aug 29.
DOI: 10.1016/j.prp.2023.154786
Abstrakt: Autophagy is a type II programmed cell death mechanism that plays a critical role in preserving cellular homeostasis through the regulation of protein, lipid, and organelle quality control. It has become gradually evident that autophagy plays a fundamental role in the initiation and progression of various types of human cancers. Nevertheless, its significance in non-melanoma skin cancers, particularly in basal cell carcinoma, has not been well documented and remains largely elusive. In this study, we aimed to illuminate the role of autophagy-associated signaling signatures during development and progression of basal cell carcinoma. For the study, a total of 72 autophagy-related genes were screened using a high-throughput qPCR approach integrating Fluidigm 96.96 Dynamic Array™ integrated fluidic circuits (IFC) and BioMark™ HD Real-Time PCR system, which enabled efficient and precise analysis of gene expression patterns. Results were analyzed using Fluidigm's Real-Time PCR Analysis software and 2 -ΔΔCt formula was used for the calculation of expression changes. Notably, expression levels of INS, TMEM74 and IFNA2 genes were identified to be prominently altered in BCC comparted to adjacent healthy tissues. However, only IFNA2 expression showed statistically significant change in BCC. Consequently, these findings suggest that IFNA2 might play significant role in the regulation of autophagy in BCC development and progression and can be therapeutically targeted.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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