Overcoming Barriers Associated with Oral Delivery of Differently Sized Fluorescent Core-Shell Silica Nanoparticles.

Autor: Erstling JA; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA.; Department of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA., Bag N; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA., Gardinier TC; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Kohle FFE; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA.; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA., DomNwachukwu N; Department of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA., Butler SD; Department of Biomedical Sciences, Cornell University, Ithaca, NY, 14853, USA., Kao T; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Ma K; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Turker MZ; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Feuer GB; Department of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA., Lee R; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Naguib N; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA.; Department of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA., Tallman JF; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Malarkey HF; Department of Applied and Engineering Physics, Cornell University, Ithaca, NY, 14853, USA., Tsaur L; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Moore WL; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Chapman DV; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Aubert T; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA., Mehta S; Center for Precision Nutrition and Health, Division of Nutritional Sciences, Cornell University, Ithaca, NY, 14853, USA., Cerione RA; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA., Weiss RS; Department of Biomedical Sciences, Cornell University, Ithaca, NY, 14853, USA., Baird BA; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA., Wiesner UB; Department of Materials Science and Engineering, Cornell University, Ithaca, NY, 14853, USA.; Kavli Institute at Cornell for Nanoscale Science, Cornell University, Ithaca, NY, 14853, USA.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2024 Jan; Vol. 36 (1), pp. e2305937. Date of Electronic Publication: 2023 Nov 23.
DOI: 10.1002/adma.202305937
Abstrakt: Oral delivery, while a highly desirable form of nanoparticle-drug administration, is limited by challenges associated with overcoming several biological barriers. Here, the authors study how fluorescent and poly(ethylene glycol)-coated (PEGylated) core-shell silica nanoparticles sized 5 to 50 nm interact with major barriers including intestinal mucus, intestinal epithelium, and stomach acid. From imaging fluorescence correlation spectroscopy studies using quasi-total internal reflection fluorescence microscopy, diffusion of nanoparticles through highly scattering mucus is progressively hindered above a critical hydrodynamic size around 20 nm. By studying Caco-2 cell monolayers mimicking the intestinal epithelia, it is observed that ultrasmall nanoparticles below 10 nm diameter (Cornell prime dots, [C' dots]) show permeabilities correlated with high absorption in humans from primarily enhanced passive passage through tight junctions. Particles above 20 nm diameter exclusively show active transport through cells. After establishing C' dot stability in artificial gastric juice, in vivo oral gavage experiments in mice demonstrate successful passage through the body followed by renal clearance without protein corona formation. Results suggest C' dots as viable candidates for oral administration to patients with a proven pathway towards clinical translation and may generate renewed interest in examining silica as a food additive and its effects on nutrition and health.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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