In vitro hyperspectral biomarkers of human chondrosarcoma cells in nanoparticle-mediated radiosensitization using carbon ions.

Autor: Tudor M; Department of Life and Environmental Physics, Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering, Reactorului 30, P.O. Box MG-6, 077125, Magurele, Romania.; Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095, Bucharest, Romania., Popescu RC; Department of Life and Environmental Physics, Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering, Reactorului 30, P.O. Box MG-6, 077125, Magurele, Romania.; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, Gheorghe Polizu Street, 1-7, 011061, Bucharest, Romania., Negoita RD; Applied Sciences Doctoral School, Politehnica University Bucharest, Bucharest, Romania., Gilbert A; UMR6252 CIMAP, Team Applications in Radiobiology with Accelerated Ions, CEA-CNRS-ENSICAEN-Université de Caen Normandie, 14000, Caen, France., Ilisanu MA; Doctoral School of Computer Sciences, Politehnica University Bucharest, Bucharest, Romania., Temelie M; Department of Life and Environmental Physics, Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering, Reactorului 30, P.O. Box MG-6, 077125, Magurele, Romania., Dinischiotu A; Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095, Bucharest, Romania. anca.dinischiotu@unibuc.ro., Chevalier F; UMR6252 CIMAP, Team Applications in Radiobiology with Accelerated Ions, CEA-CNRS-ENSICAEN-Université de Caen Normandie, 14000, Caen, France., Mihailescu M; Holographic Imaging and Processing Laboratory, Physics Department, Politehnica University Bucharest, Bucharest, Romania.; Centre for Research in Fundamental Sciences Applied in Engineering, Politehnica University Bucharest, Bucharest, Romania., Savu DI; Department of Life and Environmental Physics, Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering, Reactorului 30, P.O. Box MG-6, 077125, Magurele, Romania. dsavu@nipne.ro.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Sep 09; Vol. 13 (1), pp. 14878. Date of Electronic Publication: 2023 Sep 09.
DOI: 10.1038/s41598-023-41991-9
Abstrakt: New therapeutic approaches are needed for the management of the highly chemo- and radioresistant chondrosarcoma (CHS). In this work, we used polyethylene glycol-encapsulated iron oxide nanoparticles for the intracellular delivery of the chemotherapeutic doxorubicin (IONP DOX ) to augment the cytotoxic effects of carbon ions in comparison to photon radiation therapy. The in vitro biological effects were investigated in SW1353 chondrosarcoma cells focusing on the following parameters: cell survival using clonogenic test, detection of micronuclei (MN) by cytokinesis blocked micronucleus assay and morphology together with spectral fingerprints of nuclei using enhanced dark-field microscopy (EDFM) assembled with a hyperspectral imaging (HI) module. The combination of IONP DOX with ion carbon or photon irradiation increased the lethal effects of irradiation alone in correlation with the induction of MN. Alterations in the hyperspectral images and spectral profiles of nuclei reflected the CHS cell biological modifications following the treatments, highlighting possible new spectroscopic markers of cancer therapy effects. These outcomes showed that the proposed combined treatment is promising in improving CHS radiotherapy.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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