Development and characterization of polydeoxyribonucleotide (PDRN) loaded chitosan polyplex: In vitro and in vivo evaluation of wound healing activity.

Autor: Dananjaya SHS; Zerone Cellvane Inc, 3(rd) Floor, Sanhak Building, Dankook University, Dandae-ro 119, Dongnam-gu, Cheonan Si, Chungcheongnam-do 31116, Republic of Korea., Madushani KGP; Department of Botany, Faculty of Natural Sciences, The Open University of Sri Lanka, Sri Lanka., Dilrukshi J; Sri Lanka Accreditation Board for Conformity Assessment Colombo, Sri Lanka., De Silva ND; Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka., Sandamalika WMG; Department of Aquaculture and Fisheries, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Sri Lanka., Kim D; Zerone Cellvane Inc, 3(rd) Floor, Sanhak Building, Dankook University, Dandae-ro 119, Dongnam-gu, Cheonan Si, Chungcheongnam-do 31116, Republic of Korea., Choi D; Zerone Cellvane Inc, 3(rd) Floor, Sanhak Building, Dankook University, Dandae-ro 119, Dongnam-gu, Cheonan Si, Chungcheongnam-do 31116, Republic of Korea., De Zoysa M; College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of Korea., Attanayake AP; Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka. Electronic address: anojaattanayake@med.ruh.ac.lk.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Dec 31; Vol. 253 (Pt 3), pp. 126729. Date of Electronic Publication: 2023 Sep 09.
DOI: 10.1016/j.ijbiomac.2023.126729
Abstrakt: Polydeoxyribonucleotide (PDRN) is an accelerated diabetic wound healing therapy with promising abilities to promote cell growth, angiogenesis, collagen synthesis, and reduce inflammation where its sustainable delivery and release behavior is critical to ensure effective wound healing properties. Therefore, a nanopolyplex was developed here, by encapsulating PDRN with chitosan to affirm its delivery systematically. The physicochemical characterization revealed its successful encapsulation which facilitates the gradual release of PDRN. In vitro studies of the polyplex demonstrated no cytotoxicity and enhanced cell proliferation and migration properties with high antimicrobial activities. In vivo, wound healing studies in Wistar rats dorsal skin defect model induced with diabetes mellitus affirm the highest wound healing activity and wound closure rate by chitosan/PDRN polyplex treatment. Considerably high histopathological changes such as epithelialization, collagen deposition, blood vessels, and hair follicle formation were observed under the polyplex treatment. The immunohistochemical analysis for platelet endothelial cell adhesion molecule (CD31) and cluster of differentiation (CD68) revealed the ability of polyplex to increase CD31 expression and decrease CD68 expression thereby promoting the wound healing process. Collectively, these results suggest that significantly accelerated, high-quality wound healing effects could be obtained by the developed chitosan/PDRN polyplex and thus it could be introduced as a potential therapeutic product for diabetic wound healing.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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