The Characterization and Evaluation of the Soluble Triggering Receptor Expressed on Myeloid Cells-like Transcript-1 in Stable Coronary Artery Disease.

Autor: Bayrón-Marrero Z; Department of Biology, University of Puerto Rico-Rio Piedras, San Juan, PR 00936, USA., Branfield S; Department of Biology, University of Puerto Rico-Rio Piedras, San Juan, PR 00936, USA.; Department of Biology, Oakland University, Rochester Hills, MI 48309, USA., Menéndez-Pérez J; Department of Biology, University of Puerto Rico-Rio Piedras, San Juan, PR 00936, USA., Nieves-López B; Department of Biology, University of Puerto Rico-Rio Piedras, San Juan, PR 00936, USA., Ospina L; Department of Biology, University of Puerto Rico-Rio Piedras, San Juan, PR 00936, USA., Cantres-Rosario Y; Translational Proteomics Center, Comprehensive Cancer Center, University of Puerto Rico, San Juan, PR 00936, USA., Melendez LM; Translational Proteomics Center, Comprehensive Cancer Center, University of Puerto Rico, San Juan, PR 00936, USA.; Department of Microbiology and Medical Zoology, University of Puerto Rico Medical Sciences Campus, San Juan, PR 00936, USA., Hunter R; Retroviral Research Center, Universidad Central del Caribe, Bayamón, PR 00960, USA., Gibson A; Division of Natural Sciences, Maryville College, Maryville, TN 37804, USA., Maldonado-Martínez G; Exercise Sciences Program, Universidad del Sagrado Corazón, San Juan, PR 00914, USA., Washington AV; Department of Biology, University of Puerto Rico-Rio Piedras, San Juan, PR 00936, USA.; Department of Biology, Oakland University, Rochester Hills, MI 48309, USA.; William Beaumont School of Medicine, Oakland University, Rochester Hills, MI 48309, USA.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Sep 04; Vol. 24 (17). Date of Electronic Publication: 2023 Sep 04.
DOI: 10.3390/ijms241713632
Abstrakt: Platelets play crucial roles in the development and progression of coronary artery disease (CAD). The triggering receptor expressed in myeloid cells-like transcript-1 (TLT-1) is stored in platelet α granules, and activated platelets release a soluble fragment (sTLT-1). We set out to better characterize the constituent amino acids of sTLT-1 and to evaluate sTLT-1 for use as a biomarker in patients with stable CAD. We evaluated sTLT-1 release using immunoprecipitation and mass spectrometry and employed statistical methods to retrospectively correlate sTLT-1 concentrations, utilizing ELISA in plasma samples from 1510 patients with documented stable CAD. We identified TLT-1 residues to 133 in platelet releasates. ADAM17 cuts TLT-1, suggesting that S136 is the C-terminal amino acid in sTLT-1. Our results revealed that for CAD patients, sTLT-1 levels did not differ significantly according to primary outcomes of death or major cardiac event; however, patients with left ventricular (LV) dysfunction had significantly lower plasma sTLT-1 levels as compared to those with normal LV function (981.62 ± 1141 pg/mL vs. 1247.48 ± 1589 pg/mL; p = 0.003). When patients were stratified based on sTLT-1 peak frequency distribution (544 pg/mL), a significant association with congestive heart failure was identified (OR = 2.94; 1.040-8.282; p = 0.042), which could be explained by LV dysfunction.
Databáze: MEDLINE
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