Magnetic amyloid-based biocatalyst for the hydrolysis of urea.

Autor: de Athayde Moncorvo Collado A; Instituto de Medicina Molecular y Celular Aplicada, Universidad Nacional de Tucumán-Consejo Nacional de Investigación Científicas y Técnicas- Sistema Provincial de Salud (UNT-CONICET-SIPROSA), Pasaje Manuel Dorrego, 1080. CP 4000. Tucumán, Argentina; Instituto Superior de Investigaciones Biológicas (INSIBIO, CONICET-UNT). Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT, Batalla de Chacabuco 461, CP 4000 Tucumán, Argentina. Electronic address: aledeathayde@gmail.com., Socías SB; Instituto de Medicina Molecular y Celular Aplicada, Universidad Nacional de Tucumán-Consejo Nacional de Investigación Científicas y Técnicas- Sistema Provincial de Salud (UNT-CONICET-SIPROSA), Pasaje Manuel Dorrego, 1080. CP 4000. Tucumán, Argentina. Electronic address: sbsocias@yahoo.com.ar., González-Lizárraga F; Instituto de Medicina Molecular y Celular Aplicada, Universidad Nacional de Tucumán-Consejo Nacional de Investigación Científicas y Técnicas- Sistema Provincial de Salud (UNT-CONICET-SIPROSA), Pasaje Manuel Dorrego, 1080. CP 4000. Tucumán, Argentina. Electronic address: mflorenciagl@hotmail.com.ar., Ploper D; Instituto de Medicina Molecular y Celular Aplicada, Universidad Nacional de Tucumán-Consejo Nacional de Investigación Científicas y Técnicas- Sistema Provincial de Salud (UNT-CONICET-SIPROSA), Pasaje Manuel Dorrego, 1080. CP 4000. Tucumán, Argentina. Electronic address: diegoploper@conicet.gov.ar., Vera Pingitore E; Instituto de Medicina Molecular y Celular Aplicada, Universidad Nacional de Tucumán-Consejo Nacional de Investigación Científicas y Técnicas- Sistema Provincial de Salud (UNT-CONICET-SIPROSA), Pasaje Manuel Dorrego, 1080. CP 4000. Tucumán, Argentina. Electronic address: estebanverapingitore@conicet.gov.ar., Chehín RN; Instituto de Medicina Molecular y Celular Aplicada, Universidad Nacional de Tucumán-Consejo Nacional de Investigación Científicas y Técnicas- Sistema Provincial de Salud (UNT-CONICET-SIPROSA), Pasaje Manuel Dorrego, 1080. CP 4000. Tucumán, Argentina; Instituto Superior de Investigaciones Biológicas (INSIBIO, CONICET-UNT). Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT, Batalla de Chacabuco 461, CP 4000 Tucumán, Argentina. Electronic address: rosana@fbqf.unt.edu.ar., Chaves S; Instituto de Medicina Molecular y Celular Aplicada, Universidad Nacional de Tucumán-Consejo Nacional de Investigación Científicas y Técnicas- Sistema Provincial de Salud (UNT-CONICET-SIPROSA), Pasaje Manuel Dorrego, 1080. CP 4000. Tucumán, Argentina. Electronic address: silvinachaves@conicet.gov.ar.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Feb 01; Vol. 433, pp. 136830. Date of Electronic Publication: 2023 Sep 03.
DOI: 10.1016/j.foodchem.2023.136830
Abstrakt: The presence of urea in wines and other alcoholic beverages represents a critical problem since it can chemically react with ethanol, which leads to the formation of ethyl carbamate, a carcinogenic agent according to the World Health Organization. Here we report the creation of a biocatalyst for the hydrolysis of urea, which could potentially be used before bottling alcoholic drinks. For this, the effective surface area of streptavidin-labeled magnetic microparticles was amplified by functionalization with biotin-labeled hen egg lysozyme amyloid fibers. Subsequently, by using copper and hydrogen peroxide induced cross-linking of unmodified proteins (CHICUP), soybean urease was immobilized to the fibers. This gave rise to a magnetic biocatalyst with remarkable urease activity, which was maintained even after 10 reuses. We propose that this strategy could be used as a platform for immobilizing other molecules to design and develop a myriad of biocatalysts for the food industry.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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