Genetic analysis of the rice jasmonate receptors reveals specialized functions for OsCOI2.

Autor: Nguyen HT; DIADE, IRD, Univ Montpellier, Montpellier, France., Cheaib M; DIADE, IRD, Univ Montpellier, Montpellier, France., Fournel M; DIADE, IRD, Univ Montpellier, Montpellier, France.; IBMP, CNRS, Univ Strasbourg, Strasbourg, France., Rios M; DIADE, IRD, Univ Montpellier, Montpellier, France., Gantet P; DIADE, IRD, Univ Montpellier, Montpellier, France., Laplaze L; DIADE, IRD, Univ Montpellier, Montpellier, France., Guyomarc'h S; DIADE, IRD, Univ Montpellier, Montpellier, France., Riemann M; Karlsruhe Institute of Technology, Botanical Institute, Karlsruhe, Germany., Heitz T; IBMP, CNRS, Univ Strasbourg, Strasbourg, France., Petitot AS; DIADE, IRD, Univ Montpellier, Montpellier, France., Champion A; DIADE, IRD, Univ Montpellier, Montpellier, France.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Sep 08; Vol. 18 (9), pp. e0291385. Date of Electronic Publication: 2023 Sep 08 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0291385
Abstrakt: COI1-mediated perception of jasmonate is critical for plant development and responses to environmental stresses. Monocots such as rice have two groups of COI genes due to gene duplication: OsCOI1a and OsCOI1b that are functionally equivalent to the dicotyledons COI1 and OsCOI2 whose function remains unclear. In order to assess the function of OsCOI2 and its functional redundancy with COI1 genes, we developed a series of rice mutants in the 3 genes OsCOI1a, OsCOI1b and OsCOI2 by CRISPR Cas9-mediated editing and characterized their phenotype and responses to jasmonate. Characterization of OsCOI2 uncovered its important roles in root, leaf and flower development. In particular, we show that crown root growth inhibition by jasmonate relies on OsCOI2 and not on OsCOI1a nor on OsCOI1b, revealing a major function for the non-canonical OsCOI2 in jasmonate-dependent control of rice root growth. Collectively, these results point to a specialized function of OsCOI2 in the regulation of plant development in rice and indicate that sub-functionalisation of jasmonate receptors has occurred in the monocot phylum.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Nguyen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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