A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry.

Autor:	Engevik KA; Department of Molecular Virology & Microbiology, Baylor College of Medicine, Houston, TX 77030, USA., Gonzalez H; Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA., Daniels C; Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA., Stavert RK; Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA., Oezguen N; Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA., Engevik MA; Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA; Department of Microbiology & Immunology, Medical University of South Carolina, Charleston, SC 29425, USA., Horvath TD; Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA; Department of Pathology, Texas Children's Hospital, Houston, TX 77030, USA. Electronic address: thomas.horvath2@bcm.edu.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2023 Sep 15; Vol. 4 (3), pp. 102540. Date of Electronic Publication: 2023 Sep 07.
DOI:	10.1016/j.xpro.2023.102540
Abstrakt:	We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial growth, collecting cell-free supernatant, litmus dye addition, and pH-based calibration curve preparations. This assay has been designed for flexible formatting that can accommodate both high-volume and low-volume sample sets. Competing Interests: Declaration of interests T.D.H. is an Editorial Advisory Board member and is contracted as an Associate Academic Editor for a Cell Press journal called STAR Protocols. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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