Sustainable Nanoparticles from Stephania glabra and Analysis of Their Anticancer Potential on 2D and 3D Models of Prostate Cancer.

Autor: Vaid P; School of Biotechnology, Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, 173229, H, Solan, .P, India., Saini AK; Central Research Laboratory and Department of Bio-sciences and Technology, MMEC, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana, 133207, India., Gupta RK; Department of Chemical Engineering, Indian Institute of Technology Kanpur, U, Kanpur, .P, India., Sinha ES; Department of Biotechnology, Panjab University, Chandigarh, 160014, India., Sharma D; CSIR-Institute of Microbial Technology, Chandigarh, 160036, India., Alsanie WF; Department of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia., Thakur VK; Biorefining and Advanced Materials Research Centre, Scotland's Rural College (SRUC), Kings Buildings, Edinburgh, EH9 3JG, UK., Saini RV; Central Research Laboratory and Department of Bio-sciences and Technology, MMEC, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana, 133207, India. reenavohra10@gmail.com.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2024 Jun; Vol. 196 (6), pp. 3511-3533. Date of Electronic Publication: 2023 Sep 08.
DOI: 10.1007/s12010-023-04700-w
Abstrakt: In pursuit of a novel effective treatment for prostate cancer, methanolic extract of Stephania glabra tubers (Sg-ME) was utilized to fabricate silver (Sg-AgNP), copper oxide (Sg-CuONP), and silver-copper bimetallic nanoparticles (Sg-BNP). The characterization of the nanoparticles confirmed spherical shape with average diameters of 30.72, 32.19, and 25.59 nm of Sg-AgNP, Sg-CuONP, and Sg-BNP, respectively. Interestingly, these nanoparticles exhibited significant cytotoxicity toward the prostate cancer (PC3) cell line while being non-toxic toward normal cells. The nanoparticles were capable of inducing apoptosis in PC3 cells by enhancing reactive oxygen species (ROS) generation and mitochondrial depolarization. Furthermore, the shrinkage of 3D prostate tumor spheroids was observed after 4 days of treatment with these green nanoparticles. The 3D model system was less susceptible to nanoparticles as compared to the 2D model system. Sg-BNP showed the highest anticancer potential on 2D and 3D prostate cancer models.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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