In vitro activity of sanitizers against mono- and polymicrobial biofilms of C. parapsilosis and S. aureus .

Autor: Castro VdP; Laboratory of Mycology and Environmental Diagnosis, Universidade de Franca , Franca, São Paulo, Brazil., Thomaz DY; Laboratory of Medical Mycology (LIM-53), Instituto de Medicina Tropical e Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo , São Paulo, Brazil., Vieira KdL; Laboratory of Mycology and Environmental Diagnosis, Universidade de Franca , Franca, São Paulo, Brazil., Lopes LG; Laboratory of Mycology and Environmental Diagnosis, Universidade de Franca , Franca, São Paulo, Brazil., Rossi F; Central Laboratory Division (LIM 03) - Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo , São Paulo, Brazil., Del Negro GMB; Laboratory of Medical Mycology (LIM-53), Instituto de Medicina Tropical e Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo , São Paulo, Brazil., Benard G; Laboratory of Medical Mycology (LIM-53), Instituto de Medicina Tropical e Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo , São Paulo, Brazil., Pires RH; Laboratory of Mycology and Environmental Diagnosis, Universidade de Franca , Franca, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2023 Oct 18; Vol. 67 (10), pp. e0053423. Date of Electronic Publication: 2023 Sep 08.
DOI: 10.1128/aac.00534-23
Abstrakt: The emergence of disinfectant-resistant microorganisms poses a significant threat to public health. These resilient pathogens can survive and thrive in hospital settings despite routine disinfection practices, leading to persistent infections and the potential for outbreaks. In this study, we investigated the impact of 11 different commercial sanitizers at various concentrations and exposure times on biofilms consisting of clinical and nosocomial environmental isolates of Candida parapsilosis and Staphylococcus aureus . Among the sanitizers tested, 0.5% and 2.0% chlorhexidine (CLX), 10% polyvinyl pyrrolidone (PVP-I), a disinfectant based on quaternary ammonium compound (QAC), 2% glutaraldehyde, and 0.55% orthophthalaldehyde (OPA) demonstrated efficacy against both C. parapsilosis and S. aureus in monospecies and mixed biofilms. Analysis showed that 0.5% CLX and 10% PVP-I had fungicidal and bactericidal activity against all biofilms. However, the sanitizer based on QAC and 0.55% OPA proved to be bacteriostatic and fungicidal against both monospecies and mixed biofilms. In mixed biofilms, despite the last four sanitizers exerting fungicidal action, the reduction of fungal cells was approximately 4 log 10 CFU/mL compared to monospecies biofilms, showing that the interaction provided more resistance of the yeast to the sanitizer. Formation of mixed biofilms in hospital settings can create an ecological niche that enhances the survival of pathogens against routine sanitization procedures. Therefore, effective sanitization practices, including regular cleaning with effective sanitizers, should be implemented to prevent C. parapsilosis/S. aureus biofilm formation in healthcare settings.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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