Iodixanol supplementation in freezing extender improves the antioxidant capacity of semen.

Autor: Marqui FN; School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Sao Paulo, Brazil., Martins A Jr; School of Veterinary Medicine, São Paulo State University (UNESP), Sao Paulo, Brazil., da Cruz TE; School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Sao Paulo, Brazil., Berton TIU; Tairana Artificial Insemination Station, São Paulo, Brazil., de Paula Freitas-Dell'Aqua C; School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Sao Paulo, Brazil., Dell'Aqua JA Jr; School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Sao Paulo, Brazil., Oba E; School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Sao Paulo, Brazil.
Jazyk: angličtina
Zdroj: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2023 Nov; Vol. 58 (11), pp. 1551-1558. Date of Electronic Publication: 2023 Sep 07.
DOI: 10.1111/rda.14470
Abstrakt: The aim of this study was to evaluate the effect of supplementing bovine semen freezing extender with different concentrations of iodixanol on post-thaw sperm characteristics. Six ejaculates of three Nellore bulls were pooled and diluted in commercial extender (BotuBov®) and then divided into 4 groups: control group (without adding iodixanol); groups G1.5, G3, or G6 according to the concentration of iodixanol solution (RedCushion®). After dilution, the samples were cooled and frozen. Post-thaw semen evaluation included sperm motility by CASA immediately after thawing and after 60 min of incubation at 37°C, flow cytometry analysis for integrity of plasma and acrosomal membranes, membrane destabilization and translocation of phosphatidylserine, mitochondrial membrane potential, and formation of intracellular anion superoxide ( O 2 - ), hydrogen peroxide (H 2 O 2 ), and membrane lipid peroxidation. The group G6 presented significantly higher (p < .05) total and progressive motility, percentage of plasma and acrosomal membrane integrity, and H 2 O 2 than control and group G1.5. Furthermore, group G6 showed lower (p < .05) lipid peroxidation than control. In addition, regardless of the concentration used, the percentage of spermatozoa without phosphatidylserine translocation was higher (p < .05) in all iodixanol supplemented groups. In conclusion, iodixanol supplementation preserved the motility and integrity of sperm membranes during cryopreservation and protected against lipid peroxidation.
(© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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