Mapping Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display.
| Autor: | Tsoumpeli MT; School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire, UK., Varghese A; School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire, UK., Owen JP; RSK-ADAS Ltd, Beeston, Nottinghamshire, UK., Maddison BC; RSK-ADAS Ltd, Beeston, Nottinghamshire, UK., Daly JM; School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire, UK., Gough KC; School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire, UK. Kevin.Gough@nottingham.ac.uk. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2702, pp. 467-487. |
| DOI: | 10.1007/978-1-0716-3381-6_25 |
| Abstrakt: | Peptide phage display has historically been used to epitope map monoclonal antibodies. More recently, by coupling this method with next-generation sequencing (so-called next-generation phage display, NGPD) to mass screen peptide binding events, the methodology has been successfully applied to map polyclonal antibody responses to infection. This leads to the identification of panels of mimotopes that represent the pathogen's epitopes. One potential advantage of using such an approach is that the mimotopes can represent not just linear epitopes but also conformational epitopes or those produced from post-translational modifications of proteins or from other non-protein macromolecules. The mapping of such complex immunological recognition of a pathogen can inform novel serological assay development and vaccine design. Here, we provide detailed methods for the application of NGPD to identify panels of mimotopes that are recognized specifically by antibodies from individuals with a particular infection. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|