Primary structural features, physicochemical and biological properties of two water-soluble polysaccharides extracted from the brown Tunisian seaweed Halopteris scoparia.

Autor: Hadjkacem F; Laboratoire de Génie Enzymatique et Microbiologie, Equipe de Biotechnologie des Algues, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, 3038 Sfax, Tunisia; Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France., Elleuch J; Laboratoire de Génie Enzymatique et Microbiologie, Equipe de Biotechnologie des Algues, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, 3038 Sfax, Tunisia., Aitouguinane M; Centre d'Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech-URL-CNRST-05), Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakech 40000, Morocco., Chakou FZ; Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria., Ursu AV; Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France., Dubessay P; Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France., Bourgougnon N; Laboratoire de Biotechnologie et Chimie Marines, Université Bretagne Sud, Lorient, France., Traikia M; Institute of Chemistry of Clermont-Ferrand, Clermont Auvergne University, CNRS, SIGMA Clermont, 63000 Clermont-Ferrand, France., Le Cerf D; Normandie Université, UNIROUEN, INSA Rouen, CNRS, PBS, 76000 Rouen, France., El Alaoui-Talibi Z; Centre d'Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech-URL-CNRST-05), Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakech 40000, Morocco., El Modafar C; Centre d'Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (Centre AgroBiotech-URL-CNRST-05), Faculté des Sciences et Techniques Marrakech, Université Cadi Ayyad, Marrakech 40000, Morocco., Boual Z; Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria., El Hadj MDO; Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria., Delattre C; Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France; Institut Universitaire de France (IUF), 1 Rue Descartes, 75005 Paris, France., Christophe G; Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France., Michaud P; Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France., Fendri I; Laboratory of Plant Biotechnologies Applied to the Improvement of Plants, Faculty of Sciences, University of Sfax, 3038 Sfax, Tunisia., Abdelkafi S; Laboratoire de Génie Enzymatique et Microbiologie, Equipe de Biotechnologie des Algues, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, 3038 Sfax, Tunisia., Pierre G; Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, France; Institut Universitaire de France (IUF), 1 Rue Descartes, 75005 Paris, France. Electronic address: guillaume.pierre@uca.fr.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Dec 31; Vol. 253 (Pt 2), pp. 126757. Date of Electronic Publication: 2023 Sep 09.
DOI: 10.1016/j.ijbiomac.2023.126757
Abstrakt: Marine algae are the most abundant resource in the marine environment and are still a promising source of bioactive compounds including hydrocolloids. This study contributes to the evaluation of the biological and biotechnological potentials of two water soluble polysaccharides, namely alginates (AHS) and fucoidan (FHS), extracted and purified from Halopteris scoparia, an abundant Tunisian brown macroalgae collected in Tunisia (Tabarka region). The total sugars, neutral monosaccharides, uronic acids, proteins, polyphenols, and sulfate groups contents were quantified for both fractions, as well as their functional groups and primary structural features by Fourier transform infrared spectroscopy, ionic and/or gas chromatography and nuclear magnetic resonance analyses. AHS and FHS showed significant anti-inflammatory (IC 50  ≈ 1 mg/mL), anticoagulant (e.g., 27-61.7 for the activated partial thromboplastin time), antihyperglycemic (0.1-40 μg/mL) and anti-trypsin (IC 50  ≈ 0.3-0.4 mg/mL) effects. FHS and a hydrolyzed fraction showed a very promising potential against herpes viruses (HSV-1) (IC 50  < 28 μg/mL). Besides, AHS and two hydrolyzed fractions were able to stimulate the natural defenses of tomato seedlings, assessing their elicitor capacity, by increasing the activity of phenylalanine ammonia-lyase (66-422 %) but also significantly changing the polyphenol content in the leaves (121-243 %) and roots (30-104 %) of tomato plants.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Guillaume Pierre reports financial support was provided by Campus France. Slim Abdelkafi reports financial support was provided by Tunisian Ministry of Higher Education and Scientific Research.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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