Age-Related Histologic and Biochemical Changes in Auricular and Nasal Cartilages.

Autor: Kim SG; University of North Carolina School of Medicine, Chapel Hill, North Carolina, U.S.A., Menapace DC; Division of Facial Plastic & Reconstructive Surgery, Mayo Clinic, Jacksonville, Florida, U.S.A., Mims MM; Division of Facial Plastic & Reconstructive Surgery, University of Oklahoma, Oklahoma City, Oklahoma, U.S.A., Shockley WW; Division of Facial Plastic & Reconstructive Surgery, University of North Carolina, Chapel Hill, North Carolina, U.S.A., Clark JM; Division of Facial Plastic & Reconstructive Surgery, University of North Carolina, Chapel Hill, North Carolina, U.S.A.
Jazyk: angličtina
Zdroj: The Laryngoscope [Laryngoscope] 2024 Mar; Vol. 134 (3), pp. 1220-1226. Date of Electronic Publication: 2023 Sep 07.
DOI: 10.1002/lary.30990
Abstrakt: Objective: Analyze age-related changes in histologic features and biochemical properties of human auricular cartilage and two subsites of nasal cartilages (quadrangular cartilage and dorsal septal articulation with upper lateral cartilages).
Study Design: Prospective cross-sectional study of nasal and auricular cartilages from seventy-three (73) live donors.
Methods: Auricular cartilage (AC), quadrangular cartilage (QC), and dorsal septal cartilage articulation (DSA) with the upper lateral cartilage (ULCs) were collected intraoperatively. Histochemical staining was used: Safranin O for glycosaminoglycans (GAGs), Verhoeff's for elastin, and Masson's trichrome for collagen. ImageJ2 software was used to calculate cell count and percent stained for each cartilage type. R studio "ggplot" package was used to visualize age versus cell count or percent stained.
Results: Participant ages ranged from 20 to 77 years, average 46.5 years. There was a significant decline in GAGs with age for the DSA subsite, (n = 64, p < 0.001). Significant increase in collagen content with age was observed for DSA subsite (n = 66, p < 0.001) and the QC subsite (n = 64, p < 0.05). There was a statistically insignificant decline in elastin with age (n = 41, p = 0.309) for AC. Cell count declined with age at all cartilage subsites.
Conclusion: Our findings confirm that there were age-related decreases in cartilage glycosaminoglycan content, and chondrocyte cell count in both auricular and nasal cartilages. We have also confirmed that collagen content increases with age for both auricular and nasal cartilage. The histologic findings while not statistically significant in all comparisons, provides additional evidence that there is some loss of structural integrity and flexibility in nasal and auricular cartilage with aging.
Level of Evidence: NA Laryngoscope, 134:1220-1226, 2024.
(© 2023 The American Laryngological, Rhinological and Otological Society, Inc.)
Databáze: MEDLINE
Externí odkaz: