Genetic and molecular analysis of stem rot (Sclerotinia sclerotiorum) resistance in Brassica napus (canola type).

Autor: Khan MA; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, Australia 6009.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, Australia 6009.; Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, 38000, Pakistan., Cowling WA; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, Australia 6009.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, Australia 6009., Banga SS; Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004, Punjab, India., Barbetti MJ; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, Australia 6009.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, Australia 6009., Cantila AY; School of Biological Sciences, The University of Western Australia, Perth, WA, Australia 6009., Amas JC; School of Biological Sciences, The University of Western Australia, Perth, WA, Australia 6009., Thomas WJW; School of Biological Sciences, The University of Western Australia, Perth, WA, Australia 6009., You MP; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, Australia 6009.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, Australia 6009., Tyagi V; Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004, Punjab, India., Bharti B; Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004, Punjab, India., Edwards D; School of Biological Sciences, The University of Western Australia, Perth, WA, Australia 6009.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, Australia 6009., Batley J; School of Biological Sciences, The University of Western Australia, Perth, WA, Australia 6009.; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, Australia 6009.
Jazyk: angličtina
Zdroj: Heliyon [Heliyon] 2023 Aug 21; Vol. 9 (9), pp. e19237. Date of Electronic Publication: 2023 Aug 21 (Print Publication: 2023).
DOI: 10.1016/j.heliyon.2023.e19237
Abstrakt: Identifying the molecular and genetic basis of resistance to Sclerotinia stem rot ( Sclerotinia sclerotiorum ) is critical for developing long-term and cost-effective management of this disease in rapeseed/canola ( Brassica napus ). Current cultural or chemical management options provide, at best, only partial and/or sporadic control. Towards this, a B. napus breeding population (Mystic x Rainbow) , including the parents, F 1 , F 2 , BC 1 P1 and BC 1 P2, was utilized in a field study to determine the inheritance pattern of Sclerotinia stem rot resistance (based on stem lesion length, SLL). Broad sense heritability was 0.58 for SLL and 0.44 for days to flowering (DTF). There was a significant negative correlation between SLL and stem diameter (SD) (r = -0.39) and between SLL and DTF (r = -0.28), suggesting co-selection of SD and DTF traits, along with SLL, should assist in improving overall resistance. Non-additive genetic variance was evident for SLL, DTF, and SD. In a genome wide association study (GWAS), a significant quantitative trait locus (QTL) was identified for SLL. Several putative candidate marker trait associations (MTA) were located within this QTL region. Overall, this study has provided valuable new understanding of inheritance of resistance to S. sclerotiorum , and has identified QTL, MTAs and transgressive segregants with high-level resistances. Together, these will foster more rapid selection for multiple traits associated with Sclerotinia stem rot resistance, by enabling breeders to make critical choices towards selecting/developing cultivars with enhanced resistance to this devastating pathogen.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2023 The Authors.)
Databáze: MEDLINE