Nanocomposite based on green synthesis of gold nanoparticles decorated with functionalized multi-walled carbon nanotubes for the electrochemical determination of hydroxychloroquine.

Autor: Nardi N; Laboratory of Biosensors - Department of Chemistry, Federal University of Santa Catarina, Campus Trindade, Florianópolis, SC 88040-900, Brazil., Baumgarten LG; Laboratory of Biosensors - Department of Chemistry, Federal University of Santa Catarina, Campus Trindade, Florianópolis, SC 88040-900, Brazil., Dreyer JP; Laboratory of Biosensors - Department of Chemistry, Federal University of Santa Catarina, Campus Trindade, Florianópolis, SC 88040-900, Brazil., Santana ER; Laboratory of Biosensors - Department of Chemistry, Federal University of Santa Catarina, Campus Trindade, Florianópolis, SC 88040-900, Brazil., Winiarski JP; Laboratory of Biosensors - Department of Chemistry, Federal University of Santa Catarina, Campus Trindade, Florianópolis, SC 88040-900, Brazil. Electronic address: joneswnk@gmail.com., Vieira IC; Laboratory of Biosensors - Department of Chemistry, Federal University of Santa Catarina, Campus Trindade, Florianópolis, SC 88040-900, Brazil. Electronic address: iolanda.vieira@ufsc.br.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2023 Nov 30; Vol. 236, pp. 115681. Date of Electronic Publication: 2023 Aug 25.
DOI: 10.1016/j.jpba.2023.115681
Abstrakt: In this study, a selective and sensitive electrochemical approach for determining hydroxychloroquine (HCQ) was proposed. A novel nanocomposite based on gold nanoparticles synthesized by green synthesis in an extract of white pitaya (Hylocereus undatus) (AuNP-Ext) decorated with functionalized multi-walled carbon nanotubes (f-MWCNTs) was presented. AuNP-Ext was characterized by ultraviolet-visible spectroscopy and the f-MWCNTs/AuNP-Ext nanocomposite by transmission electron microscopy. The nanocomposite was used to modify a glassy carbon electrode (GCE). Using the f-MWCNT-AuNP-Ext/GCE sensor, an irreversible oxidation peak at +0.74 V vs. Ag/AgCl was verified by HCQ. The calibration plot was studied in two linear ranges, from 0.03 to 3.5 µmol/L and from 3.5 to 17.0 µmol/L, with a limit of detection of 0.0093 µmol/L and a limit of quantification of 0.031 µmol/L, regarding the first linear range. The proposed sensor was successfully applied to the determination of HCQ in pharmaceutical and clinical samples without any special purification, separation or pre-treatment steps. The accuracy was verified by UV-Vis spectrometry, and this revealed that the proposed method was accurate and precise, as evidenced by F- and t-tests.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: