Mapping antibody footprints using binding profiles.

Autor:	Azulay A; The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, Beer-Sheva, Israel.; National Institute of Biotechnology in the Negev, Beer-Sheva, Israel., Cohen-Lavi L; National Institute of Biotechnology in the Negev, Beer-Sheva, Israel.; Department of Industrial Engineering and Management, Ben-Gurion University of the Negev, Beer-Sheva, Israel., Friedman LM; The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, Beer-Sheva, Israel.; National Institute of Biotechnology in the Negev, Beer-Sheva, Israel., McGargill MA; Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA., Hertz T; The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, Beer-Sheva, Israel.; National Institute of Biotechnology in the Negev, Beer-Sheva, Israel.; Vaccine and Infectious Disease Division, Fred Hutch Cancer Research Center, Seattle, WA, USA.
Jazyk:	angličtina
Zdroj:	Cell reports methods [Cell Rep Methods] 2023 Aug 22; Vol. 3 (8), pp. 100566. Date of Electronic Publication: 2023 Aug 22 (Print Publication: 2023).
DOI:	10.1016/j.crmeth.2023.100566
Abstrakt:	The increasing use of monoclonal antibodies (mAbs) in biology and medicine necessitates efficient methods for characterizing their binding epitopes. Here, we developed a high-throughput antibody footprinting method based on binding profiles. We used an antigen microarray to profile 23 human anti-influenza hemagglutinin (HA) mAbs using HA proteins of 43 human influenza strains isolated between 1918 and 2018. We showed that the mAb's binding profile can be used to characterize its influenza subtype specificity, binding region, and binding site. We present mAb-Patch-an epitope prediction method that is based on a mAb's binding profile and the 3D structure of its antigen. mAb-Patch was evaluated using four mAbs with known solved mAb-HA structures. mAb-Patch identifies over 67% of the true epitope when considering only 50-60 positions along the antigen. Our work provides proof of concept for utilizing antibody binding profiles to screen large panels of mAbs and to down-select antibodies for further functional studies. Competing Interests: The authors declare no competing interests. (© 2023 The Authors.)
Databáze:	MEDLINE
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