| Autor: |
Wang B; School of Biological Engineering, Dalian Polytechnic University, Ganjingziqu, Dalian 116034, P. R. China., Zhao X; School of Biological Engineering, Dalian Polytechnic University, Ganjingziqu, Dalian 116034, P. R. China., Fu T; School of Biological Engineering, Dalian Polytechnic University, Ganjingziqu, Dalian 116034, P. R. China., Chen X; School of Biological Engineering, Dalian Polytechnic University, Ganjingziqu, Dalian 116034, P. R. China., Guo X; School of Biological Engineering, Dalian Polytechnic University, Ganjingziqu, Dalian 116034, P. R. China., Li X; School of Biological Engineering, Dalian Polytechnic University, Ganjingziqu, Dalian 116034, P. R. China., Yang F; School of Biological Engineering, Dalian Polytechnic University, Ganjingziqu, Dalian 116034, P. R. China. |
| Abstrakt: |
Promoters are essential for designing Saccharomyces cerevisiae cell factories. Identifying novel promoters tuned to produce specific metabolites under increasingly diverse industrial stresses is required to improve the economic feasibility of whole fermentation processes. In this study, a positively evolved Suc2 promoter (SUC 2p) with promoter activity stronger than that of the wild-type Suc2 promoter (SUC 2wtp) was obtained. Quantitative real-time PCR, fluorescence analysis, Western blotting, and a β-galactosidase activity assay revealed that SUC 2p is a medium-strength promoter compared with eight reported promoters at a medium glucose concentration (2% (w/v)). Different glucose concentrations modulated the strength of SUC 2p. Low glucose concentrations (0.05 and 0.5% (w/v)) enhanced the promoter strength of SUC 2p dramatically, with promoter activity higher than that of reported strong promoters. Glucose starvation resulted in the formation of a new Msn2/4 binding site on SUC 2p. Our work should facilitate the development of promoters with novel fine-tuning properties and the construction of S. cerevisiae cell factories suitable for the industrial production of essential chemicals under glucose-deprived conditions. |
