| Autor: |
Vinueza-Burgos C; Unidad de Investigación en Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Universidad Central del Ecuador, Quito, Ecuador., Medina-Santana J; Unidad de Investigación en Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Universidad Central del Ecuador, Quito, Ecuador., Maldonado R; Laboratorio de Sanidad Animal Agencia de Regulación y Control Fito y Zoosanitario, Quito, Ecuador., Vásquez Y; Laboratorio de Bacteriología y Micología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador., Lincango L; Laboratorio de Bacteriología y Micología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador., Villagomez E; Laboratorio de Bacteriología y Micología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador., Gómez C; Laboratorio de Bacteriología y Micología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador., Ron-Garrido L; Centro Internacional de Zoonosis, Universidad Central del Ecuador, Quito, Ecuador., Cevallos-Almeida MB; Laboratorio de Bacteriología y Micología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador. |
| Abstrakt: |
Salmonella Infantis and Enteritidis serovars have been reported as important causes of salmonellosis in humans worldwide. However, the virulence of these two serovars has yet to be compared. To evaluate the virulence of Salmonella Infantis ( n = 23) and Salmonella Enteritidis ( n = 7), we used two models: the Caco2 cells model ( in vitro ) and the Galleria mellonella model ( in vivo ). Additionally, the virulence genes of all tested strains were contrasted with phenotypic outcomes. Results showed that adhesion means were 18.2% for Salmonella Enteritidis and 38.2% for Salmonella Infantis strains. Invasion means were 77.1% for Salmonella Enteritidis and 56.2% for Salmonella Infantis strains. Significant differences were found between serovars in adherence and invasion assays. Mortality rates (58% for Salmonella Enteritidis and 62.6% for Salmonella Infantis) were not significantly different between serotypes. The distribution of virulence genes showed that genes fae (fimbrial adherence determinants) and shdA (nonfimbrial adherence determinants) were only found in Salmonella Infantis strains. On the other hand, the rck gene (invasion) and Plasmid-encoded fimbriae genes ( pef A , B , C , D ) were present in Salmonella Enteritidis exclusively. In conclusion, this study shows that S almonella Enteritidis has a higher virulence potential under experimental conditions than Salmonella Infantis. However, more studies are needed to determine the risk that Salmonella Infantis could represent compared with Salmonella Enteritidis. Moreover, other in vivo models should be considered to assess the virulence of these serovars. |
