Effects of prenatal Doppler ultrasound on the retina of the chick embryo in ovo.

Autor: König J; Department of Anesthesia and Intensive Care, University Hospital Rostock, Rostock, Germany., Rahn A; Department of Pediatrics, Rostock University Medical Center, Rostock, Germany., Schätzel J; Department of Ophthalmology, Rostock University Medical Center, Rostock, Germany., Frank M; Medical Biology and Electron Microscopy Centre, Rostock University Medical Center, Rostock, Germany.; Department of Life, Light and Matter, University of Rostock, Rostock, Germany., Stahnke T; Institute for Implant Technology and Biomaterials-IIB e.V., Rostock, Germany., Witt M; Department of Anatomy, Rostock University Medical Center, Rostock, Germany.; Department of Anatomy, Medical Faculty, Technische Universität Dresden, Dresden, Germany., Stachs A; Department of Obstetrics and Gynecology, Rostock University Medical Center, Rostock, Germany., Stachs O; Department of Ophthalmology, Rostock University Medical Center, Rostock, Germany.; Department of Life, Light and Matter, University of Rostock, Rostock, Germany., Langner I; Department of Orthopedics and Orthopedic Surgery, University Medicine Greifswald, Greifswald, Germany., Lindner T; Core Facility Multimodal Small Animal Imaging, Rostock University Medical Center, Rostock, Germany.; Institute for Diagnostic and Interventional Radiology, Pediatric and Neuroradiology, Rostock University Medical Center, Rostock, Germany., Beller E; Institute for Diagnostic and Interventional Radiology, Pediatric and Neuroradiology, Rostock University Medical Center, Rostock, Germany., Fischer DC; Department of Pediatrics, Rostock University Medical Center, Rostock, Germany., Streckenbach F; Institute for Diagnostic and Interventional Radiology, Pediatric and Neuroradiology, Rostock University Medical Center, Rostock, Germany.
Jazyk: angličtina
Zdroj: Anatomia, histologia, embryologia [Anat Histol Embryol] 2023 Nov; Vol. 52 (6), pp. 1003-1009. Date of Electronic Publication: 2023 Sep 05.
DOI: 10.1111/ahe.12960
Abstrakt: Currently, the effect of prenatal ultrasound on foetal development is intensively discussed and the guidelines for prenatal diagnostics have been changed. However, data supporting these concerns are scarce. Therefore, we used an established in ovo model of the chicken embryo to investigate cell proliferation and apoptosis within the retina. A total of 21 chicken eggs were fenestrated on Day 5 and allocated to either the control (n = 8) or exposition group (n = 13). The exposition group was treated with pulsed-wave Doppler ultrasound (PWD) for 10 min while controls remained without treatment. After subsequent incubation (6-48 h), the eggs were sacrificed, and chicken embryos were examined morphologically (HE-staining) and immunohistochemically. Counting of apoptotic and proliferating cells per retina was performed using antibodies specific for phospho-histone-H3 and active caspase-3 in combination with a biotin-labelled secondary antibody and peroxidase conjugated avidin-biotin complex for chromogenic detection. Due to a rather low number of specimens at each time point after ultrasound exposition, we neglected the effects of incubation time and focused on treatment effects. This approach revealed that the median number of proliferating cells is reduced after 10 min of exposure to PWD (569 vs. 766), while the number of apoptotic cells is fairly comparable between groups (5 vs. 6). Our data contribute to a better understanding of prenatal US on foetal development by suggesting that PWD could have an impact on the number of proliferating cells in the developing chicken retina and therefore justify further investigations.
(© 2023 The Authors. Anatomia, Histologia, Embryologia published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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