The genetic and physiological basis of Arabidopsis thaliana tolerance to Pseudomonas viridiflava.

Autor: Duque-Jaramillo A; Max Planck Institute for Biology Tübingen, Tübingen, 72076, Germany., Ulmer N; Max Planck Institute for Biology Tübingen, Tübingen, 72076, Germany., Alseekh S; Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, 14476, Germany., Bezrukov I; Max Planck Institute for Biology Tübingen, Tübingen, 72076, Germany., Fernie AR; Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, 14476, Germany., Skirycz A; Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, 14476, Germany.; Boyce Thompson Institute, Cornell University, Ithaca, 14850, USA., Karasov TL; Max Planck Institute for Biology Tübingen, Tübingen, 72076, Germany.; School of Biological Sciences, University of Utah, Salt Lake City, 84112, USA., Weigel D; Max Planck Institute for Biology Tübingen, Tübingen, 72076, Germany.; Institute for Bioinformatics and Medical Informatics, University of Tübingen, Tübingen, 72074, Germany.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2023 Dec; Vol. 240 (5), pp. 1961-1975. Date of Electronic Publication: 2023 Sep 04.
DOI: 10.1111/nph.19241
Abstrakt: The opportunistic pathogen Pseudomonas viridiflava colonizes > 50 agricultural crop species and is the most common Pseudomonas in the phyllosphere of European Arabidopsis thaliana populations. Belonging to the P. syringae complex, it is genetically and phenotypically distinct from well-characterized P. syringae sensu stricto. Despite its prevalence, we lack knowledge of how A. thaliana responds to its native isolates at the molecular level. Here, we characterize the host response in an A. thaliana - P. viridiflava pathosystem. We measured host and pathogen growth in axenic infections and used immune mutants, transcriptomics, and metabolomics to determine defense pathways influencing susceptibility to P. viridiflava infection. Infection with P. viridiflava increased jasmonic acid (JA) levels and the expression of ethylene defense pathway marker genes. The immune response in a susceptible host accession was delayed compared with a tolerant one. Mechanical injury rescued susceptibility, consistent with an involvement of JA. The JA/ethylene pathway is important for suppression of P. viridiflava, yet suppression capacity varies between accessions. Our results shed light on how A. thaliana can suppress the ever-present P. viridiflava, but further studies are needed to understand how P. viridiflava evades this suppression to spread broadly across A. thaliana populations.
(© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.)
Databáze: MEDLINE
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