Ectopic callose deposition into woody biomass modulates the nano-architecture of macrofibrils.

Autor: Bourdon M; The Sainsbury Laboratory, University of Cambridge, Cambridge, UK. mat2hieu.bourdon@gmail.com.; Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland. mat2hieu.bourdon@gmail.com., Lyczakowski JJ; Department of Biochemistry, University of Cambridge, Cambridge, UK.; Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland., Cresswell R; Department of Physics, University of Warwick, Coventry, UK., Amsbury S; Centre for Plant Science, Faculty of Biological Sciences, University of Leeds, Leeds, UK.; Plants, Photosynthesis and Soil, School of Biosciences, The University of Sheffield, Sheffield, UK., Vilaplana F; Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, Stockholm, Sweden.; Wallenberg Wood Science Centre (WWSC), KTH Royal Institute of Technology, Stockholm, Sweden., Le Guen MJ; Scion, Te Papa Tipu Innovation Park, Rotorua, New Zealand., Follain N; Normandie Université, UNIROUEN Normandie, INSA Rouen, CNRS, PBS, Rouen, France., Wightman R; The Sainsbury Laboratory, University of Cambridge, Cambridge, UK., Su C; Wood Development Group, University of Helsinki, Helsinki, Finland., Alatorre-Cobos F; The Sainsbury Laboratory, University of Cambridge, Cambridge, UK.; Conacyt-Unidad de Bioquimica y Biologia Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Mexico., Ritter M; Wood Materials Science, Institute for Building Materials, ETH Zürich, Zürich, Switzerland.; Empa Wood Tec, Cellulose and Wood Materials Laboratory, Dübendorf, Switzerland., Liszka A; Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.; Doctoral School of Exact and Natural Sciences, Jagiellonian University, Krakow, Poland., Terrett OM; Department of Biochemistry, University of Cambridge, Cambridge, UK., Yadav SR; Wood Development Group, University of Helsinki, Helsinki, Finland.; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India., Vatén A; Wood Development Group, University of Helsinki, Helsinki, Finland.; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences and Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland.; Stomatal Development and Plasticity group, University of Helsinki, Helsinki, Finland., Nieminen K; Wood Development Group, University of Helsinki, Helsinki, Finland.; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences and Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland.; Production systems / Tree Breeding Department, Natural Resources Institute Finland (Luke), Helsinki, Finland., Eswaran G; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences and Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland., Alonso-Serra J; Wood Development Group, University of Helsinki, Helsinki, Finland.; UMR 5667 Reproduction et Développement Des Plantes, ENS de Lyon, France., Müller KH; Cambridge Advanced Imaging Centre, Department of Physiology, Development and Neuroscience, Cambridge, UK., Iuga D; Department of Physics, University of Warwick, Coventry, UK., Miskolczi PC; Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden., Kalmbach L; The Sainsbury Laboratory, University of Cambridge, Cambridge, UK.; Molecular Plant Physiology, Institute of Biology II, University of Freiburg, Freiburg, Germany., Otero S; The Sainsbury Laboratory, University of Cambridge, Cambridge, UK.; Science and Technology Office of the Congress of Deputies, Madrid, Spain., Mähönen AP; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences and Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland., Bhalerao R; Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden., Bulone V; Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, Stockholm, Sweden.; College of Medicine and Public Health, Flinders University, Bedford Park, South Australia, Australia., Mansfield SD; Department of Wood Science, Faculty of Forestry, University of British Columbia, Vancouver, British Columbia, Canada.; Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada., Hill S; Scion, Te Papa Tipu Innovation Park, Rotorua, New Zealand., Burgert I; Wood Materials Science, Institute for Building Materials, ETH Zürich, Zürich, Switzerland.; Empa Wood Tec, Cellulose and Wood Materials Laboratory, Dübendorf, Switzerland., Beaugrand J; Biopolymères Interactions Assemblages (BIA), INRA, Nantes, France., Benitez-Alfonso Y; The Centre for Plant Science, The Bragg Centre, The Astbury Centre, University of Leeds, Leeds, UK., Dupree R; Department of Physics, University of Warwick, Coventry, UK., Dupree P; Department of Biochemistry, University of Cambridge, Cambridge, UK. pd101@cam.ac.uk., Helariutta Y; The Sainsbury Laboratory, University of Cambridge, Cambridge, UK. yrjo.helariutta@helsinki.fi.; Wood Development Group, University of Helsinki, Helsinki, Finland. yrjo.helariutta@helsinki.fi.; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences and Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland. yrjo.helariutta@helsinki.fi.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2023 Sep; Vol. 9 (9), pp. 1530-1546. Date of Electronic Publication: 2023 Sep 04.
DOI: 10.1038/s41477-023-01459-0
Abstrakt: Plant biomass plays an increasingly important role in the circular bioeconomy, replacing non-renewable fossil resources. Genetic engineering of this lignocellulosic biomass could benefit biorefinery transformation chains by lowering economic and technological barriers to industrial processing. However, previous efforts have mostly targeted the major constituents of woody biomass: cellulose, hemicellulose and lignin. Here we report the engineering of wood structure through the introduction of callose, a polysaccharide novel to most secondary cell walls. Our multiscale analysis of genetically engineered poplar trees shows that callose deposition modulates cell wall porosity, water and lignin contents and increases the lignin-cellulose distance, ultimately resulting in substantially decreased biomass recalcitrance. We provide a model of the wood cell wall nano-architecture engineered to accommodate the hydrated callose inclusions. Ectopic polymer introduction into biomass manifests in new physico-chemical properties and offers new avenues when considering lignocellulose engineering.
(© 2023. The Author(s).)
Databáze: MEDLINE
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