Anti-proliferative activity, molecular genetics, docking analysis, and computational calculations of uracil cellulosic aldehyde derivatives.

Autor: Fahim AM; Green Chemistry Department, National Research Centre (NRC), P.O. Box 12622, DokkiCairo, Egypt. am.abdel-wahid@nrc.sci.eg., Dacrory S; Cellulose and Paper Department, National Research Centre, P.O. Box 12622, Giza, Egypt., Elsayed GH; Department of Hormones, National Research Centre (NRC), P.O. Box 12622, Dokki, Giza, Egypt.; Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre (NRC), P.O. Box 1262, Dokki, Giza, Egypt.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Sep 04; Vol. 13 (1), pp. 14563. Date of Electronic Publication: 2023 Sep 04.
DOI: 10.1038/s41598-023-41528-0
Abstrakt: In this study, the oxidation of microcrystalline cellulose using NaIO 4 to yield the corresponding cellulose aldehyde utilized microwave irradiation as a green tool, the obtained cellulosic aldehyde was confirmed through spectral analysis and it has an active site to react with the synthesized uracil acetamide to afford the corresponding arylidene cellulosic MDAU(4), the latter compound which can easily due to presence of active CH=group behind a cyano group react with nitrogen nucleophile's and cyclized with hydrazine hydrate to give pyrazole cellulosic MDPA(5). The spectral analysis of the obtained cellulosic derivatives was confirmed with FT-IR, NMR, and SEM. Additionally, a neutral red uptake analysis has been used to investigate the cytotoxic activity of the cellulosic compounds MDAC(2), MDAU(4), and MDAP(5) against the cancer cells A549 and Caco2. After 48 h, Compound MDAU(4) had a stronger inhibitory effect on the growth of A549 and Caco2, compared to standard values. Then, using QRT-PCR, the appearance sites of the genes -Catenin, c-Myc, Cyclin D1, and MMP7 in A549 cells were examined. By reducing the expression levels of the Wnt signaling cascade genes -Catenin, c-Myc, Cyclin D1, and MMP7 when administered to A549 cells, compound MDAU(4) was shown in this investigation to be a viable candidate compared to lung cancer. Additionally, docking simulation was used to explore the uracil cellulosic heterocycles attached to different proteins, and computational investigations of these compounds looked at how well their physical characteristics matched the outcomes of their experiments.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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